Effects of Hydrogen Sulfide on Bacterial Communities on the Surface of Galatheid Crab, Shinkaia crosnieri, and in a Bacterial Mat Cultured in Rearing Tanks

• Konishi Masaaki

  Institute of Biogeoscience (Biogeos), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

• Watsuji Tomo-o

  Institute of Biogeoscience (Biogeos), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

• Nakagawa Satoshi

  Institute of Biogeoscience (Biogeos), Japan Agency for Marine-Earth Science and Technology (JAMSTEC) Faculty of Fisheries Sciences, Hokkaido University

• Hatada Yuji

  Institute of Biogeoscience (Biogeos), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

• Takai Ken

  Institute of Biogeoscience (Biogeos), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

• Toyofuku Takashi

  Institute of Biogeoscience (Biogeos), Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

書誌事項

タイトル別名

• Effects of Hydrogen Sulfide on Bacterial Communities on the Surface of Galatheid Crab, <i>Shinkaia crosnieri</i>, and in a Bacterial Mat Cultured in Rearing Tanks

抄録

To investigate the effects of H₂S on the bacterial consortia on the galatheid crab, Shinkaia crosnieri, crabs of this species were cultivated in the laboratory under two different conditions, with and without hydrogen sulfide feeding. We developed a novel rearing tank system equipped with a feedback controller using a semiconductor sensor for hydrogen sulfide feeding. H₂S aqueous concentration was successfully maintained between 5 to 40 μM for 80 d with the exception of brief periods of mechanical issues. According to real-time PCR analysis, the numbers of copies of partial 16S rRNA gene of an episymbiont of the crabs with H₂S feeding was three orders of magnitude larger than that without feeding. By phylogenetic analysis of partial 16S rRNA gene, we detected several clones related to symbionts of deep sea organisms in Alphaproteobacteria, Gammaproteobacteria, Epsilonproteobacteria, and Flavobacteria, from a crab with H₂S feeding. The symbiont-related clones were grouped into four different groups: Gammaproteobacteria in marine epibiont group I, Sulfurovum-affiliated Epsilonproteobacteria, Osedax mucofloris endosymbiont-affiliated Epsilonproteobacteria, and Flavobacteria closely related to CFB group bacterial epibiont of Rimicaris exoculata. The other phylotypes were related to Roseobacter, and some Flavobacteria, seemed to be free-living psychrophiles. Furthermore, white biofilm occurred on the surface of the rearing tank with H₂S feeding. The biofilms contained various phylotypes of Gammaproteobacteria, Epsilonproteobacteria, and Flavobacteria, as determined by phylogenetic analysis. Interestingly, major clones were related to symbionts of Alviniconcha sp. type 2 and to endosymbionts of Osedax mucofloris, in Epsilonproteobacteria.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 28 (1), 25-32, 2013

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• hydrogen sulfide
• epibiont
• symbiont
• tank
• feedback control

詳細情報

• CRID: 1390282679323880960
• NII論文ID: 10031138540
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me12070
• COI: 1:STN:280:DC%2BC3s%2FmsVyitA%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 024298035
• PubMed: 23080406
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024298035
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/28/1/28_ME12070/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可