Uranium (U)-Tolerant Bacterial Diversity from U Ore Deposit of Domiasiat in North-East India and Its Prospective Utilisation in Bioremediation

• Kumar Rakshak

  Microbiology Laboratory, Department of Biotechnology & Bioinformatics, North-Eastern Hill University

• Nongkhlaw Macmillan

  Microbiology Laboratory, Department of Biotechnology & Bioinformatics, North-Eastern Hill University

• Acharya Celin

  Molecular Biology Division, BioMedical Group, Bhabha Atomic Research Centre

• Joshi Santa Ram

  Microbiology Laboratory, Department of Biotechnology & Bioinformatics, North-Eastern Hill University

抄録

Uranium (U)-tolerant aerobic chemo-heterotrophic bacteria were isolated from the sub-surface soils of U-rich deposits in Domiasiat, North East India. The bacterial community explored at molecular level by amplified ribosomal DNA restriction analysis (ARDRA) resulted in 51 distinct phylotypes. Bacterial community assemblages at the U mining site with the concentration of U ranging from 20 to 100 ppm, were found to be most diverse. Representative bacteria analysed by 16S rRNA gene sequencing were affiliated to Firmicutes (51%), Gammaproteobacteria (26%), Actinobacteria (11%), Bacteroidetes (10%) and Betaproteobacteria (2%). Representative strains removed more than 90% and 53% of U from 100 μM and 2 mM uranyl nitrate solutions, respectively, at pH 3.5 within 10 min of exposure and the activity was retained until 24 h. Overall, 76% of characterized isolates possessed phosphatase enzyme and 53% had P_IB-type ATPase genes. This study generated baseline information on the diverse indigenous U-tolerant bacteria which could serve as an indicator to estimate the environmental impact expected to be caused by mining in the future. Also, these natural isolates efficient in uranium binding and harbouring phosphatase enzyme and metal-transporting genes could possibly play a vital role in the bioremediation of metal-/radionuclide-contaminated environments.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 28 (1), 33-41, 2013

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• Domiasiat bacterial diversity
• uranium tolerance and binding
• phosphatase
• P_IB-type ATPase gene
• bioremediation

詳細情報

• CRID: 1390001204347169152
• NII論文ID: 10031138541
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me12074
• COI: 1:STN:280:DC%2BC3s%2FmsVyitQ%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 024298045
• PubMed: 23080407
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024298045
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/28/1/28_ME12074/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可