Bacterial Population Dynamics in a Laboratory Activated Sludge Reactor Monitored by Pyrosequencing of 16S rRNA

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  • Satoh Hiroyasu
    Department of Socio-cultural and Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo
  • Oshima Kenshiro
    Center for Omics and Bioinformatics, Graduate School of Frontier Sciences, The University of Tokyo
  • Suda Wataru
    Center for Omics and Bioinformatics, Graduate School of Frontier Sciences, The University of Tokyo
  • Ranasinghe Purnika
    Department of Socio-cultural and Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo
  • Li Ning
    Department of Socio-cultural and Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo
  • Gunawardana Egodaha Gedara Wasana
    Department of Socio-cultural and Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo
  • Hattori Masahira
    Center for Omics and Bioinformatics, Graduate School of Frontier Sciences, The University of Tokyo
  • Mino Takashi
    Department of Socio-cultural and Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo

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The microbial population in a laboratory activated sludge reactor was monitored for 245 d at 75 time points by pyrosequencing of 16S rRNA. Synthetic wastewater was used as the influent, and the reactor was operated under the same conditions throughout the experiment. The behaviors of different bacterial operational taxonomic units (OTUs) were observed. Multiple OTUs showed periodic propagation and recession. One of the OTUs showed sharp recession, which suggests that cells in the OTU were selectively killed. The behaviors of different phylogenetic lineages of Candidatus ‘Accumulibacter phosphatis’ were also visualized. It was clearly demonstrated that pyrosequencing with barcoded primers is a very effective tool to clarify the dynamics of the bacterial population in activated sludge.<br>

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