Migration of Mononuclear Cells Expressing β-Actin Through the Adventitia into Media and Intima in Coronary Arteriogenesis and Venogenesis in Ischemic Myocardium
-
- Uchida Yasuto
- Department of Cardiology, Toho University Medical Center Ohmori Hospital
-
- Uchida Yasumi
- Japan Foundation for Cardiovascular Research Department of Cardiology, The Jikei University School of Medicine
-
- Maezawa Yoshiro
- Sick Children’s Hospital
-
- Maezawa Yuko
- Samuel Lunenfeld Institute, Mount Sinai Hospital
-
- Tabata Tsuyoshi
- Department of Clinical Physiology, Toho University Medical Center Sakura Hospital
この論文をさがす
抄録
It was previously thought that arteriogenesis and venogenesis are induced not only by proliferation of vessel-resident smooth muscle cells (SMCs) and endothelial cells (ECs) but also by migration of their precursors. However, it is not well understood through what route(s) the precursors migrate into the existing vessels.<br>We examined through what route or routes circulating mononuclear cells expressing β-actin (β-MNCs), which we identified in canine coronary vessels, migrate into coronary vessel walls and cause arteriogenesis and venogenesis at 1, 2, 4 and 8 weeks after induction of myocardial infarction.<br>The following changes were observed: (1) The β-MNCs migrated via coronary microvessels to the interstitial space at one week; (2) β-MNCs traversed the adventitia into the media and settled in parallel with pre-existing smooth muscle cells (SMCs) in arterioles and arteries and lost β-actin and acquired α-smooth muscle actin (α-SMA) to become mature SMCs at 2-4 weeks; (3) at the same time, other β-MNCs migrated across the adventitia and media into the intima and settled in parallel with pre-existing endothelial cells (ECs) and lost β-actin, while acquiring CD31, to become mature ECs, resulting in arteriogenesis; (4) Similarly, β-MNCs migrated into venular and venous walls and became SMCs or ECs, resulting in venogenesis.<br>β-MNCs in the interstitial space expressed CD34 but not other major vascular cell markers.<br>β-MNCs, possibly a vascular progenitor, migrate not from the lumen but across the adventitia into the media or intima of coronary vessels and transit to SMCs or ECs, and participate in arteriogenesis and venogenesis in ischemic myocardium.
収録刊行物
-
- International Heart Journal
-
International Heart Journal 53 (1), 54-63, 2012
一般社団法人 インターナショナル・ハート・ジャーナル刊行会