ゲニステインとエイコサペンタエン酸によるヒト乳癌細胞増殖抑制における相乗効果

• 中川 宏之

  関西医大病理学第2講座 関西医大外科学第2講座

• 蔦 幸治

  関西医大病理学第2講座

• 仙崎 英人

  関西医大病理学第2講座

• 植村 芳子

  関西医大病理学第2講座

• 清塚 康彦

  関西医大病理学第2講座

• 四方 伸明

  関西医大病理学第2講座

• 田中 完児

  関西医大外科学第2講座

• 日置 紘士郎

  関西医大外科学第2講座

• 螺良 愛郎

  関西医大病理学第2講座

書誌事項

タイトル別名

• Synergistic effects of genistein and eicosapentaenoic acid on the growth inhibition of the human breast cancer cell lines

抄録

Asiand iet is characteristic of its highc ontento f soy productsa nd large amountso f fat derivedf rom fish oil leadingto lowr isk of breastc anceri n Asianst han in Westernw omen. Epidemiologsitcu diesh aves hownt hat the consumptioonf soy in Asianw omenh as beena ssociatedw ith decreasedra tes of canceri ncludingb reast cancer. Soyc ontainsl arge amountso f isoflavonesa, nd amongth em, genisteinis one of the mosta ctiveg rowthi nhibitor in a variety of tumors. On the other hand, the diet of Greenland Eskimos contains a high content of the ω-3polyunsaturated fatty acid such as eicosapentaenoic acid (EPA) known to be present in fish oil, which is related to low risk of breast cancer. In this regard, effect of genistein or EPA on tumor growth suppression in vitro was evaluated using human breast cancer cell lines (MCF-7 and MDA-MB-231). Then, the mode of cell death was evaluated by FCM. Finally, the synergism between genistein and EPA was investigated. Genistein stimulated estrogen receptor (ER)-positive MCF-7 cells at low concentrations while produced a dose- and time-dependent in vitro growth inhibition at high concentrations. EPA caused dose- and time-dependent growth inhibition. Apoptosis was confirmed by the appearance of sub G₁ fraction in both cell lines. Genistein exerted synergism with EPA on MCF-7 cells (genistein > 25.2μg/ml and EPA > 65.9μg/ml) and on MDA-MB-231 cells(genistein > 47.6μg/ml and EPA > 190.4μg/ml). Thus the Asian dietary habit is beneficial for suppressing breast cencer growth that genistein in combination with EPA may be promising agent in breast cancer suppression.

収録刊行物

• Progress in Oncology

  Progress in Oncology 10 (1), 19-23, 2000

  近畿脳腫瘍病理検討会

キーワード

• breast cancer
• genistein
• eicosapentaenoic acid
• apoptosis
• synergism

詳細情報

• CRID: 1390001205420821120
• NII論文ID: 130001933714
• DOI: 10.11452/neurooncology1991.10.1_19
• ISSN: 21870543
• ISSN: 09176969
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可