高速液体クロマトグラフィーによるコルチコイドの分析第2報, 合成ステロイド剤服用者の血液および尿中ステロイドの分離

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  • The High Pressure Liquid Chromatography of Corticoids II. Analysis of Synthetic Corticoids in Blood and Urine
  • 高速液体クロマトグラフィーによるコルチコイドの分析-2-合成ステロイド剤服用者の血液および尿中ステロイドの分離
  • コウソク エキタイ クロマトグラフィー ニヨル コルチコイド ノ ブンセキ 2

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The high pressure liquid chromatographic (HPLC) technique was developed to separate and quantitate the synthetic corticosteroids (s-CS) which are widely used clinically. 1) 12 kinds of s-CS in alcoholic solvent and 2) some of their metabolites in the plasma and urine of healthy subjects with oral administration of s-CS were investigated for the preliminary work. <BR>The results are summarized as follows : <BR>1) Cortisol sodium phosphate, Dexamethasone 21, disodium phosphate, Paramethasone acetate, Cortisol acetate, Cortisone acetate, Methylprednisolone acetate, Prednisone, Dexamethasone, 9α-fluorocortisol, Betamethasone, Triamcinolone, and Prednisolone in ethanol were clearly separated by HPLC from Cortisol (F). In the suitable condition of the HPLC (LC-2 type) with a Zorbax SIL column, organic solvent (cyclohexane : dichloromethane : ethanol = 9 : 4 : 1) -carrier mobile phases and UV detector, the retention time of each s-CS was obviously different from that of F. The calibration curve was obtained in a linear line with regards to each s-CS. The mean recovery was 97.6% and the coefficient of variation were 1.6 (intraassay) and 7.2 (interassay) %. The sensitivity of the steroid determination was 200pg order. <BR>2) The serial changes in plasma concentrations of s-CS; CS-metabolites and endogenous F were shown in 3 healthy males and 2 females following oral administration of the s-CS. The separated metabolites in number and quality depended on the kind of s-CS. Prednisone and other kinds of the acidified products were separated from prednisolone in the plasma and urinary samples of the healthy subjects as well as Addisonian patients. <BR>In conclusion, the HPLC method is useful for the separation and quantitation of the UV-absorbing CS of human plasma and urine. The obtained chromatograms may be an indication of the metabolic state of the subject with treatment of s-CS.

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