Spin-Trapping Studies on the Reaction of Iron Complexes with Peroxides and the Effects of Water-Soluble Antioxidants.
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- Kohno Masahiro
- ESR Application Laboratory, Analytical Instruments Division, JEOL Ltd.
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- Yamada Masako
- ESR Application Laboratory, Analytical Instruments Division, JEOL Ltd.
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- Mitsuta Keiichi
- ESR Application Laboratory, Analytical Instruments Division, JEOL Ltd.
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- Mizuta Yokio
- ESR Application Laboratory, Analytical Instruments Division, JEOL Ltd.
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- Yoshikawa Toshikazu
- First Department of Medicine, Kyoto Prefectural University of Medicine
抄録
Using ESR spin-trapping techniques we measured the levels of free radical species generated from six different systems (hypoxanthine-xanthine oxidase, iron(II)–hydrogen peroxide, iron(III)–hydrogen peroxide, iron(II)–t-butyl hydroperoxide, iron(III)–t-butyl hydroperoxide, and catalase (CAT)–t-butyl hydroperoxide). Six types of radicals (O2−·,·OH, ·H, ·CH3, (CH3)3CO·, and (CH3)3COO·) were detected as spin adducts of spin traps 5,5-dimethylpyrroline 1-oxide (DMPO) or 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS). Quantitative analysis of the levels of generated radicals by means of an ESR instrument also presents important information regarding the reduction of peroxides [hydrogen peroxide (H2O2) or t-butyl hydroperoxide (ROOH)] by iron(II) or iron(III) as well as catalase. In addition, the scavenging potencies of different water-soluble antioxidants such as L-ascorbic, D-isoascorbic, gallic, sorbic, and protocatechuic acids were evaluated in terms of their ability to reduce the peaks of spin adducts.
収録刊行物
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- Bulletin of the Chemical Society of Japan
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Bulletin of the Chemical Society of Japan 64 (5), 1447-1453, 1991
公益社団法人 日本化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679092991232
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- NII論文ID
- 130001978848
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- ISSN
- 13480634
- 00092673
- http://id.crossref.org/issn/00092673
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可