Immunology : Epitope Mapping of a Monoclonal Antibody against the Gp85 of Avian Leukosis Virus Subgroup J
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- SUN Miao
- College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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- YU Duo
- College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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- MO Hongfei
- College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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- CAO Hong
- College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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- CHEN Chen
- College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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- CHEN Fuyong
- College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
書誌事項
- タイトル別名
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- Epitope Mapping of a Monoclonal Antibody against the Gp85 of Avian Leukosis Virus Subgroup J
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抄録
Avian leukosis virus subgroup J poses a great threat to the poultry industry in China. To reduce the economic losses, a quick method for detection of ALV-J antigen is required for diagnosis and identification of the congenitally transmitting hens. In this study, we report the production and evaluation of one monoclonal antibody (MAb) suitable for achieving these goals. The gp85 gene of avian leukosis virus subgroup J CAUHM01 China isolates was subcloned into the expression vectors pGEX-6p-1 and pET28a and successfully expressed in E. coli. After immunizing BALB/c mice with recombinant His-Jgp85 protein, splenic cells from immunized mice were fused with SP2/0 myeloma cells to produce hybridomas. We isolated and characterized one ALV-J gp85-specific MAb by determining its titer, affinity and IgG subclass. In addition, we performed epitope mapping and determined the epitope for the MAb 1E3 to be 81-92 aa of ALV-J gp85 protein (LPWDPQELDILG). Bioinformatics analysis and IFA studies revealed that this epitope is conserved among all ALV-J isolates and that this antibody could serve as a useful reagent for ALV-J detection and diagnosis.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 74 (6), 693-697, 2012
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206430431744
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- NII論文ID
- 130002090070
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC38zpslWiuw%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 023864161
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- PubMed
- 22214862
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可