Factors Contributing to the Resistivity of a Higher Casein Diet against Choline Deficiency-Induced Hyperhomocysteinemia in Rats
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- LIU Yi-qun
- The United Graduate School of Agricultural Science, Gifu University
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- LIU Ying
- The United Graduate School of Agricultural Science, Gifu University
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- MORITA Tatsuya
- Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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- MORI Makoto
- Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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- SUGIYAMA Kimio
- Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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抄録
The mechanism by which feeding a higher casein diet results in resistance to choline deprivation-induced hyperhomocysteinemia was investigated in rats. Plasma homocysteine concentration was significantly lower in rats fed a 30% casein diet (30C) than in rats fed a 10% casein diet (10C). Choline deprivation did not enhance plasma homocysteine concentration in rats fed 30C, while it significantly enhanced plasma homocysteine concentration in rats fed 10C. The choline deprivation-induced enhancement of plasma homocysteine concentration in rats fed 10C was significantly suppressed by methionine supplementation in a dose-dependent manner in the range of 0.1 to 0.3%, but the suppressive effect of methionine became smaller with an increase in supplementation level in the range of 0.3 to 0.5%. At a 0.5% supplementation level, methionine did not exhibit any suppressive effect on choline deprivation-induced hyperhomocysteinemia. The higher plasma homocysteine concentration in rats fed choline-deprived 10C+0.5% methionine was significantly decreased by concurrent supplementation with 0.32% glycine+0.94% serine to the level of rats fed 10C. Raising dietary total amino acid level by adding 3.61% branched-chain amino acids (BCAA)+4.5% acidic amino acids (AAA) to choline-deprived 10C+0.5% methionine+0.32% glycine+0.94% serine resulted in a further decrease in plasma homocysteine concentration to a level lower than the level in rats fed 10C. Choline deprivation-induced increases in hepatic S-adenosylhomocysteine and homocysteine concentrations were significantly suppressed by supplementation with glycine+serine and further suppressed by BCAA+AAA. Hepatic cystathionine β-synthase activity and its gene expression were significantly increased by BCAA+AAA. Hepatic triglyceride concentration changed in a manner similar to that of plasma homocysteine concentration. The results indicate that there are at least three factors contributing to the resistivity of rats fed a higher casein diet (30C) to choline deprivation-induced hyperhomocysteinemia, i.e., higher intake of methionine, higher intake of glycine and serine, and higher intake of other amino acids such as BCAA and AAA.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 58 (2), 78-87, 2012
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詳細情報 詳細情報について
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- CRID
- 1390001206325716992
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- NII論文ID
- 130002103035
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- NII書誌ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BC38XmsFymt74%3D
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 023599497
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- PubMed
- 22790565
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可