Single Camera System for Multi-Parametric Fluorescence Imaging of the Heart
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- Yamanaka Takeshi
- Department of Precision Engineering, School of Engineering, The University of Tokyo
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- Arafune Tatsuhiko
- Department of Precision Engineering, School of Engineering, The University of Tokyo
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- Shibata Nitaro
- Shinjuku Mitsui Building Clinic
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- Honjo Haruo
- Research Institute of Environmental Medicine, Nagoya University
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- Kamiya Kaichiro
- Research Institute of Environmental Medicine, Nagoya University
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- Kodama Itsuo
- Research Institute of Environmental Medicine, Nagoya University
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- Sakuma Ichiro
- Department of Precision Engineering, School of Engineering, The University of Tokyo
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抄録
Optical mapping has become a typical method in cardiac electrophysiology. A single parameter is typically mapped, and recently dual mapping systems have been reported that record fluorescence signal of membrane potential and intracellular calcium using two cameras and a dichroic mirror. There are additional pairs of cardiac parameters measured by dual mapping system. However, the method to measure more than three parameters does not exist. To exploit the full potential of fluorescence imaging, an innovative method to measure multiple, more than three parameters is needed. In this study, we present new optical mapping system which records multiple, potentially more than three parameters using a single camera. The system consists of one camera, custom optical lens system, and filter wheel. The optical lens system is designed to focus the fluorescence light at filter position, and form an image on camera’s CMOS sensor. Filter wheel is rotated by a motor, which allows filter switching corresponding with needed wavelength. The camera exposure and filter switching are synchronized by phase locked loop techniques. We validated system performance by simple experiments; lens resolution, quantity of light, and synchronization. Using this system, we observed membrane potential and intracellular calcium in Langendorff perfused rabbit heart.
収録刊行物
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- Journal of Arrhythmia
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Journal of Arrhythmia 27 (Supplement), OP05_3-, 2011
日本不整脈学会
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詳細情報 詳細情報について
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- CRID
- 1390282680222421888
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- NII論文ID
- 130002130444
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- ISSN
- 18832148
- 18804276
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可