Measurement of Hippocampal Neural Activity by Radiotelemetry in Unrestrained Piglets

• SAITO Toshiyuki

  Physiology and Genetic Regulation Department, National Institute of Agrobiological Sciences

• FUJIWARA Sei-etsu

  Physiology and Genetic Regulation Department, National Institute of Agrobiological Sciences Graduate School of Science and Engineering, Yamagata University

• SASAKI Yumetaro

  Graduate School of Science and Engineering, Yamagata University

• NIWA Koichi

  Research Institute for Electronic Science, Hokkaido University

• NEMOTO Tetsu

  School of Health Science, Faculty of Medicine, Kanazawa University

• KASUYA Etsuko

  Physiology and Genetic Regulation Department, National Institute of Agrobiological Sciences

• SAKUMOTO Ryosuke

  Physiology and Genetic Regulation Department, National Institute of Agrobiological Sciences

• YAMAGUCHI Takashi

  Graduate School of Science and Engineering, Yamagata University

抄録

We modified a wireless system to measure hippocampal neural activity in pigs, and tested a handmade microdrive for adjusting the tip of the electrode. Under general anesthesia with halothane inhalation we stereotaxically placed tungsten electrodes (5 MΩ) fitted with handmade microdrives in the temporal hippocampus of male Landrace piglets, and fixed a high-input-resistance transmitter (10 MΩ; 1000-fold amplification) to the skull. Oscillation and saturation were evident in the recording system for several days after surgery. When these phenomena ceased, we successfully recorded the hippocampal electrical activity in four of eight piglets. At 5 or 6 days following surgery, hippocampal electrical activity ‹0.15 mV in amplitude was observed in resting piglets during the daytime. In this recording, delta (1.0-3.9 Hz) and theta (4.0-7.9 Hz) waves with large amplitude were frequently predominant. However, these activities often alternated with high-frequency and low-amplitude activity, even while piglets were lying down. Manipulation of the microdrive enabled us to reposition the electrodes in the hippocampus 1 week after surgery. This technical development maintains the electrode within the hippocampus to enable neuronal activity with behavioral correlates to be determined in unrestrained piglets.

収録刊行物

• Japan Agricultural Research Quarterly: JARQ

  Japan Agricultural Research Quarterly: JARQ 43 (3), 247-254, 2009

  国立研究開発法人　国際農林水産業研究センター

キーワード

• delta
• fast Fourier transformation
• <I>Sus scrofa domesticus</I>
• theta

詳細情報

• CRID: 1390001204702529536
• NII論文ID: 130002147839
• DOI: 10.6090/jarq.43.247
• ISSN: 21858896
• ISSN: 00213551
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可