Vesicular Glutamate Transporter 2 Expression in the Rat Pineal Gland: Detailed Analysis of Expression Pattern and Regulatory Mechanism

• YOSHIDA Sachine

  Kuroda Research Unit for Affiliative Social Behavior, RIKEN Brain Science Institute Laboratory of Neuroendocrinology, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• HISANO Setsuji

  Laboratory of Neuroendocrinology, Graduate School of Comprehensive Human Sciences, University of Tsukuba

抄録

Melatonin, a hormone secreted by the pineal gland, is closely related physiologically to circadian rhythm, sleep and reproduction, and also psychiatrically to mood disorders in humans. Under circadian control, melatonin secretion is modulated via nocturnal autonomic (adrenergic) stimulation to the gland, which expresses vesicular glutamate transporter (VGLUT) 1, VGLUT2 and a VGLUT1 splice variant (VGLUT1v), glutamatergic markers. Expression of VGLUT2 gene and protein in the intact gland has been reported to exhibit a rhythmic change during a day. To study VGLUT2 expression is under adrenergic control, we here performed an in vitro experiment using dispersed pineal cells of rats. Stimulation of either β-adrenergic receptor or cAMP production to the pineal cells was shown to increase mRNA level of VGLUT2, but not VGLUT1 and VGLUT1v. Because an ability of glutamate to inhibit melatonin production was previously reported in the cultured gland, it is likely that pineal VGLUT2 transports glutamate engaged in the inhibition of melatonin production.

収録刊行物

• Ｋａｎｓｅｉ　Ｅｎｇｉｎｅｅｒｉｎｇ　Ｉｎｔｅｒｎａｔｉｏｎａｌ　Ｊｏｕｒｎａｌ

  Ｋａｎｓｅｉ　Ｅｎｇｉｎｅｅｒｉｎｇ　Ｉｎｔｅｒｎａｔｉｏｎａｌ　Ｊｏｕｒｎａｌ 11 133-137, 2012

  日本感性工学会

キーワード

• Pineal gland
• Melatonin
• Vesicular glutamate transporter
• Circadian rhythm

詳細情報

• CRID: 1390001205325452288
• NII論文ID: 130002148649
• DOI: 10.5057/kei.11.133
• BIBCODE: 2012KEIJ...11..133Y
• ISSN: 21857865
• ISSN: 18840841
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可