尿酸分析用液体クロマトカラム充填剤の選択

• 入山 啓治

  東京慈恵会医科大学共同利用研究部分析機器室

• 岩本 武夫

  東京慈恵会医科大学共同利用研究部分析機器室

• 吉浦 昌彦

  東京慈恵会医科大学共同利用研究部分析機器室

書誌事項

タイトル別名

• Selection of liquid chromatographic adsorbents for the selection and determination of uric acid in biological samples

抄録

Selection of liquid chromatographic adsorbents was studied for the separation and determination of uric acid in body fluids and mammalian tissues. A solution containing norepinephrine, epinephrine, dopamine, ascorbic acid and uric acid was used as a test solution for optimizing chromatographic conditions. Eluate from a column was electrochemically monitored by aid of an electrochemical detector set at +800mV vs Ag/Ag Cl. Five column systems were examined for the complete separation of catecholamines, ascorbic acid and uric acid. All the column systems examined in this study allowed the determination of uric acid in body fluids such as serum, cerebrospinal fluid and urine under the present chromatographic conditions. The reversedphase column systems examined in this study have been found to allow the quantitative determination of uric acid in mammalian tissues as well as in body fluids.

収録刊行物

• Uric acid research

  Uric acid research 9 (1), 1-9, 1985

  一般社団法人 日本痛風・核酸代謝学会

詳細情報

• CRID: 1390282679743957888
• NII論文ID: 130003354895
• DOI: 10.14867/gnam1977.9.1_1
• ISSN: 21870098
• ISSN: 03884120
• Web Site: https://search.jamas.or.jp/link/ui/1987040562
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可