7. セルローズアセテート膜上における酸性粘液多糖の染色性に関する研究

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  • Study on Staining Properties of Acid Mucopolysaccharides on Cellulose Acetate Sheet

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The mode of staining reactions of several AMPS to alcian blue and toluidine blue O was observed on cellulose acetate strips as well as in test tubes.<BR>1. Alcian blue could stain 7 AMPS tested in all buffer conditions except that at pH 7. 0, which indicated its significant validity for qualitative and crude separation procedures.<BR>2. Toluidine blue O stained heparin in reddish violet with a strong metachromasia but chondroitin sulfate A, B and C in blue-violet color with a slight metachromatic property, and heparitin monosulfate as well as keratan sulfate in blue color without any metachromasia.<BR>Hyaluronic acid, however, resulted in differnt reactions by different commercial batches of toluidine blue O, that is, one batch did not react with this material, whereas another did positively.

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