蛍光検出高速液体クロマトグラフィ (カラムスイッチングシステム) による血清中レチノール測定法
書誌事項
- タイトル別名
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- Determination of Retinol in Serum by High-Performance Liquid Chromatography with Fluorometric Detection Using a Column-Switching System
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抄録
We developed a high-performance liquid chromatographic method with fluorometric detection using a column-switching system for the determination of retinol levels in human serum. The lower limit of detection of retinol in serum was 30 ng/ml. Retinol in sample solutions was stable at room temperature in room lighting for at least 30 h. The recovery of retinol in serum was 99% over the range 19.6 to 196 ng/0.2ml serum. Withinand between-assay reproducibilities (CV) for samples containing three concentrations of retinol (low, medium and high) were 0.8, 1.4 and 1.1% (n=10 each), and 5.0, 2.3 and 1.8% (n=5 each), respectively. A high degree of correlation was present between serum levels of retinol as determined using the present method and those determined using the conventional method (r=0.95, Syx=61, n=35).
収録刊行物
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- Japanese Journal of Clinical Chemistry
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Japanese Journal of Clinical Chemistry 22 (3), 190-194, 1993
一般社団法人 日本臨床化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679887758464
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- NII論文ID
- 130003357735
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- COI
- 1:CAS:528:DyaK2cXkvFaiuw%3D%3D
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- ISSN
- 21874077
- 03705633
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可