Dynorphin-kappa opioid receptor signaling partly mediates estrogen negative feedback effect on LH pulse in female rats

• MOSTARI Mst. Parvin

  Laboratory of Reproductive Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• IEDA Nahoko

  Laboratory of Reproductive Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• DEURA Chikaya

  Laboratory of Reproductive Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• MINABE Shiori

  Laboratory of Reproductive Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• YAMADA Shunji

  Laboratory of Reproductive Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• UENOYAMA Yoshihisa

  Laboratory of Reproductive Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

• MAEDA Kei-ichiro

  Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan

• TSUKAMURA Hiroko

  Laboratory of Reproductive Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

書誌事項

タイトル別名

• Dynorphin-Kappa Opioid Receptor Signaling Partly Mediates Estrogen Negative Feedback Effect on LH Pulses in Female Rats

抄録

Accumulating evidence suggests that the arcuate nucleus (ARC) kisspeptin/neurokinin B (NKB)/dynorphin (KNDy) neurons play a role in estrogen negative feedback action on pulsatile gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH) release. The present study aimed to determine if dynorphin (Dyn) is involved in estrogen negative feedback on pulsatile GnRH/LH release. The effect of the injection of nor-binaltorphimine (nor-BNI), a kappa-opioid receptor (KOR) antagonist, into the third cerebroventricle (3V) on LH pulses was determined in ovariectomized (OVX) adult female rats with/without replacement of negative feedback levels of estradiol (low E₂). The mean LH concentrations and baseline levels of LH secretion in nor-BNI-injected, low E₂-treated rats were significantly higher compared with vehicle-treated controls. On the other hand, the nor-BNI treatment failed to affect any LH pulse parameters in OVX rats without low E₂ treatment. These results suggest that Dyn is involved in the estrogen negative feedback regulation of pulsatile GnRH/LH release. The low E₂ treatment had no significant effect on the numbers of ARC Pdyn (Dyn gene)-,Kiss1- and Tac2 (NKB gene)-expressing cells. The treatment also did not affect mRNA levels of Pdyn and Oprk1 (KOR gene) in the ARC-median eminence region, but significantly increased the ARC kisspeptin immunoreactivity. These findings suggest that the negative feedback level of estrogen suppresses kisspeptin release from the ARC KNDy neurons through an unknown mechanism without affecting the Dyn and KOR expressions in the ARC. Taken together, the present result suggests that Dyn-KOR signaling is a part of estrogen negative feedback action on GnRH/LH pulses by reducing the kisspeptin release in female rats.

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 59 (3), 266-272, 2013

  公益社団法人 日本繁殖生物学会

キーワード

• Arcuate nucleus
• GnRH
• Kisspeptin
• Neurokinin B

詳細情報

• CRID: 1390282681314513152
• NII論文ID: 10031184756
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.2012-193
• COI: 1:STN:280:DC%2BC3szmsFKitQ%3D%3D
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 024616838
• PubMed: 23391862
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024616838
• Web Site: https://www.jstage.jst.go.jp/article/jrd/59/3/59_2012-193/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014070656
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • PubMed
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