High-Yield Expression of Mouse Aos1-Uba2-Fusion SUMO-Activating Enzyme, mAU, in a Baculovirus-Insect Cell System

• KANEMARU Ayumi

  Faculty of Sciences, Kumamoto University

• SAITOH Hisato

  Faculty of Sciences, Kumamoto University Department of New Frontier Sciences, Graduate School of Science and Technology, Kumamoto University

抄録

Small ubiquitin-related modifier (SUMO) research requires large amounts of SUMO-activating enzyme (SUMO-E1) for in vitro SUMOylation assays. In this study the expression and purification of a hexahistidine-tagged mouse Aos1-Uba2-fusion protein (His₆-mAU) in a baculovirus-insect cell system revealed that approximately 1.0 mg of highly-purified His₆-mAU was obtained from 100 mL of Sf9 cell culture. The recombinant protein had SUMO-E1 activity in multiple in vitro SUMOylation assays.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (7), 1575-1578, 2013

  公益社団法人 日本農芸化学会

キーワード

• small ubiquitin-related modifier (SUMO)
• SUMO-activating enzyme (SUMO-E1)
• baculovirus-insect cell system
• protein expression
• post-translational protein modification

詳細情報

• CRID: 1390282681455619840
• NII論文ID: 10031190472
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.130070
• COI: 1:STN:280:DC%2BC3sjos1aqsQ%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024723801
• PubMed: 23832333
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024723801
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.130070
• Web Site: https://search.jamas.or.jp/link/ui/2014095545
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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