Formulation of a Universal First-Order Rate Constant for Enzymatic Reactions

• IMOTO Taiji

  Emeritus Professor, Graduate School of Pharmaceutical Sciences, Kyushu University

抄録

It is a common practice to employ k_cat[E]₀/K_m as a first-order rate constant for the analysis of an enzymatic reaction, where [E]₀ is the total enzyme concentration. I describe in this report a serious shortcoming in analyzing enzymatic reactions when k_cat[E]₀/K_m is employed and show that k_cat[E]₀/K_m can only be applied under very limited conditions. I consequently propose the use of a more universal first-order rate constant, k_cat[ES]_K/[S]₀, where [ES]_K is the initial equilibrium concentration of the ES-complex derived from [E]₀, [S]₀ and K_m. Employing k_cat[ES]_K/[S]₀ as the first-order rate constant enables all enzymatic reactions to be reasonably simulated under a wide range of conditions, and the catalytic and binding contributions to the rate constant of any enzyme can be determined under any and all conditions.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (8), 1703-1708, 2013

  公益社団法人 日本農芸化学会

キーワード

• binding contribution
• catalytic contribution
• enzyme catalysis
• kinetics
• <i>K</i>_m

詳細情報

• CRID: 1390282681455470848
• NII論文ID: 10031195820
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.130270
• COI: 1:STN:280:DC%2BC3sfns1OltQ%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024791817
• PubMed: 23924733
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024791817
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.130270
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可