Purification and Characterization of UDP-Arabinopyranose Mutase from Chlamydomonas reinhardtii

• KOTANI Ayana

  Faculty of Agriculture, University of the Ryukyus

• TSUJI Maki

  Faculty of Agriculture, University of the Ryukyus

• AZAMA Yasushi

  Faculty of Agriculture, University of the Ryukyus

• ISHII Tadashi

  Forestry and Forest Products Research Institute

• TAKEDA Takumi

  Iwate Biotechnology Research Center

• YAMASHITA Tetsuro

  Faculty of Agriculture, Iwate University

• SHIMOJIMA Mie

  Tokyo Institute of Technology, Center for Biological Resources and Informatics

• KONISHI Teruko

  Faculty of Agriculture, University of the Ryukyus

書誌事項

タイトル別名

• Purification and Characterization of UDP-Arabinopyranose Mutase from <i>Chlamydomonas reinhardtii</i>

抄録

Chlamydomonas reinhardtii cells are surrounded by a mixture of hydroxyprolin-rich glycoproteins consisting of L-arabinose, D-galactose, D-glucose, and D-mannose residues. The L-arabinose residue is thought to be attached by a transfer of UDP-L-arabinofuranose (UDP-Araf), which is produced from UDP-L-arabinopyranose (UDP-Arap) by UDP-arabinopyranose mutase (UAM). UAM was purified from the cytosol to determine the involvement of C. reinhardtii UAM (CrUAM) in glycoprotein synthesis. CrUAM was purified 94-fold to electrophoretic homogeneity by hydrophobic and size-exclusion chromatography. CrUAM catalyzed the reversible conversion between UDP-Arap and UDP-Araf and exhibited autoglycosylation activity when UDP-D-[¹⁴C]glucose was added as substrate. Compared to the properties of native and recombinant CrUAM overexpressed in Escherichia coli, native CrUAM showed a higher affinity for UDP-Arap than recombinant CrUAM did. This increased affinity for UDP-Arap might have been caused by post-translational modifications that occur in eukaryotes but not in prokaryotes.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (9), 1874-1878, 2013

  公益社団法人 日本農芸化学会

キーワード

• cell wall
• UDP-arabinose
• UDP-arabinopyranose mutase
• reversively glycosylated polypeptide
• <i>Chlamydomonas</i>

詳細情報

• CRID: 1390282681455362688
• NII論文ID: 10031202737
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.130302
• COI: 1:STN:280:DC%2BC3sbmsFamtw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024897582
• PubMed: 24018663
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024897582
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.130302
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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