書誌事項
- タイトル別名
-
- Determination of Cortisol and Cortisone in Human Saliva Using Dispersive Liquid-liquid Microextraction Coupled to HPLC Analysis
- ヒト ダエキ チュウ コルチゾール オヨビ コルチゾン ノ ブンサンエキエキ マイクロ チュウシュツ/HPLC ブンセキ
この論文をさがす
抄録
We examined the determination of cortisol and cortisone in human saliva with dispersive liquid-liquid microextraction (DLLME) coupled to HPLC/UV detection. In this study, by adding a mixture of acetonitrile (disperser solvent) and 1,1,2,2-tetrachloroethane (extraction solvent) into the sample, DLLME was performed. The resulting sedimented phase was injected into a reversed-phase HPLC column using a mixture of water, acetonitrile, and trifluoroacetic acid as a mobile phase for isocratic elution, and the analytes were determined with an ultraviolet detector at 254 nm. The enrichment factors of this method were ca. 20 and 40 for cortisol and cortisone, respectively, and the limits of detection were 0.30 ng mL−1 and 0.20 ng mL−1, respectively. We showed that the proposed method can be used to analyze trace amounts of cortisone in human saliva samples with sufficient sensitivity.
収録刊行物
-
- 分析化学
-
分析化学 62 (8), 719-723, 2013
公益社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679638203520
-
- NII論文ID
- 10031178770
-
- NII書誌ID
- AN00222633
-
- COI
- 1:CAS:528:DC%2BC3sXhsVWiu7%2FL
-
- NDL書誌ID
- 024835383
-
- ISSN
- 05251931
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可