血中のA型肝炎ウイルス粒子には脂質膜エンベロープが存在する

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  • Circulating hepatitis A virus particles have a lipid-associated envelope
  • ケッチュウ ノ Aガタ カンエンウイルス リュウシ ニワ シシツマク エンベロープ ガ ソンザイ スル

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We addressed a presumption that hepatitis A virus (HAV) particles in the blood circulation might be lipid-associated, and have significantly lower density and larger size than so far described in textbook. Sera from patients with acute hepatitis A served as materials. In CsCl density gradient ultracentrifugation, the serum-derived HAV particles banded initially at a density of 1.17 g/ml, but shifted to a heavier density of 1.31 g/ml when treated with protease (Pronase) plus lipid solvent (chloroform) or detergent (NP40). In parallel, the sizes of HAV particles also shifted from 50-80 nm to 30-50 nm in diameter by these treatments. The heavier HAV particles (1.31 g/ml) could bind to anti-HAV antibodies, while those with a low density (1.17 g/ml) could not. Conversely, the low density (1.17 g/ml) HAV particles could bind to anti-CD59 antibodies as well as some lectins, but the high density (1.31 g/ml) ones could not. These results suggested that HAV, despite taxonomically classified as a "nonenveloped" virus, exists in the blood of infected hosts as though being an "enveloped" virus, possessing a lipid membrane-like coat, which masks viral antigens from circulating antibodies.<br>

収録刊行物

  • 肝臓

    肝臓 54 (6), 396-398, 2013

    一般社団法人　日本肝臓学会

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