Levels of IL-17, RANKL and OPG in Gingival Crevicular Fluid Obtained from Patients with Severe Orthodontic Root Resorption

• Aihara Norihito

  Department of Orthodontics, Nihon UniversitySchool of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Yamaguchi Masaru

  Department of Orthodontics, Nihon UniversitySchool of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Yamada Kunihiko

  Department of Orthodontics, Nihon UniversitySchool of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Yoshino Tomokazu

  Department of Orthodontics, Nihon UniversitySchool of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Goseki Takemi

  Department of Orthodontics, Nihon UniversitySchool of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Kasai Kazutaka

  Department of Orthodontics, Nihon UniversitySchool of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

書誌事項

タイトル別名

• <b>Levels of IL-17, RANKL and OPG in Gingival Crevicular Fluid Obtained from Patients with Severe Orthodontic Root Resorption </b>

抄録

The aim of this studywas to investigate how interleukin (IL)-17 and the receptor activator of nuclear factor kappaB (RANK) ligand (RANKL) contribute to root resorption during orthodontic tooth movement. The first objective was to determine the levels of interleukin-17 (IL-17), soluble RANKL (sRANKL) and osteoprotegerin (OPG) in gingival crevicular fluid (GCF) obtained from a patient with severe root resorption after orthodontic treatment. A second objective was to investigate the effects of IL-17 on the release of sRANKL and OPG from human periodontal ligament (hPDL) cells. Subjects with severe root resorption (>1/3 of original root length) were identified. Control group subjects with no loss of root structure while undergoing orthodontic treatment were also identified. GCF was collected non-invasivelyfrom the mesial and distal sides of each of the four upper incisors using filter paper strips. Eluted GCF was used for analysis by enzyme-linked immunosorbent assay (ELISA). Furthermore, this in vitro studywas performed to examine the effects of IL-17 on RANKL and OPG release from human PDL cells using ELISA. Concentrations of IL-17, sRANKL, sRANKL/OPG ratio in GCF were significantlyhigher in subjects with severe root resorption than in those with no resorption. In the in vitro study,IL-17 increased the release of sRANKL, and decreased that of OPG from hPDL cells in a time- and dose-dependent manner. These results suggest that IL-17 stimulates orthodonticallyinduced inflammatoryroot resorption (OIIRR) via production of RANKL in PDL.

収録刊行物

• ＩＪＯＭＳ

  ＩＪＯＭＳ 12 (1), 54-60, 2013

  日本大学松戸歯学部 口腔科学研究所

キーワード

• IL-17, RANKL, root resorption,orthodontic tooth movement,odontoclastogenesis

詳細情報

• CRID: 1390282680191171712
• NII論文ID: 130003375200
• DOI: 10.5466/ijoms.12.54
• ISSN: 21854254
• ISSN: 13479733
• Web Site: https://www.jstage.jst.go.jp/article/ijoms/12/1/12_54/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014375644
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
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