Expressions of apoptosis-regulating factors in bovine retained placenta

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  • ウシにおける胎盤停滞と胎盤節組織のアポトーシスの関係
  • ウシ ニ オケル タイバン テイタイ ト タイバンセツ ソシキ ノ アポトーシス ノ カンケイ

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Abstract

Retention of fetal membranes (RFM) is a major problem in bovine gynecology and obstetrics often leading to subsequent infertility and culling of animals. To date, endogenous and exogenous factors have been suggested to relate RFM, such as age, spices, genetic factors, environments, hormones and nutrition. However, the cause of RFM is still clearly unknown. The aim of the present study was to evaluate the relationship between RFM and apoptosis of the cells in fetal membranes. The present study investigated mRNA and protein expressions of apoptosis-regulating factors: FAS, cellular FLICE-like inhibiting protein (cFLIP), BAX, BCL2, caspase-8 (CASP8), and CASP3 in fetal membranes. FAS mRNA expression in maternal placental tissue was less in RFM cows than in non-RFM cows (p<0.05). cFLIP mRNA expression in maternal and fetal placental tissue was greater in RFM cows than in non-RFM cows (p<0.05). CASP3 mRNA expression in maternal placental tissue was greater in RFM cows than in non-RFM cows (p<0.05). In the immunohistochemical study, single-stranded DNA, which appears specifically in the apoptotic cells, was mainly found in the maternal placenta of non-RFM cows. Together these results suggest that RFM occurs at least in part due to a dysfunctional apoptotic process caused by the inhibition of FAS expression in the maternal placenta, and the increase of cFLIP expression in the maternal and fetal placenta.

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