Composition of Hemagglutinin and Neuraminidase Affects the Antigen Yield of Influenza A(H1N1)pdm09 Candidate Vaccine Viruses

• Shirakura Masayuki

  Influenza Virus Research Center, National Institute of Infectious Diseases

• Kawaguchi Akira

  Influenza Virus Research Center, National Institute of Infectious Diseases

• Tashiro Masato

  Influenza Virus Research Center, National Institute of Infectious Diseases

• Nobusawa Eri

  Influenza Virus Research Center, National Institute of Infectious Diseases

抄録

To improve the hemagglutinin (HA) antigen yield of influenza A(H1N1)pdm09 candidate vaccine viruses, we generated 7:1, 6:2, and 5:3 genetic reassortant viruses between wild-type (H1N1)pdm09 (A/California/7/2009) (Cal7) and a high-yielding master virus, A/Puerto Rico/8/34 (PR8). These viruses contained the HA; HA and neuraminidase (NA); and HA, NA, and M genes, respectively, derived from Cal7, on a PR8 backbone. The influence of the amino acid residue at position 223 in Cal7 HA on virus growth and HA antigen yield differed between these reassortant viruses. NIIDRG-7, a 7:1 virus possessing arginine at position 223, exhibited a 10-fold higher 50% egg infectious dose (EID₅₀) (10.0 log₁₀EID₅₀/ml) than the 5:3 and 6:2 viruses. It also had 1.5- to 3-fold higher protein (13.8 μg/ml of allantoic fluids) and HA antigen (4.1 μg/ml of allantoic fluids) yields than the 5:3 and 6:2 viruses, which possessed identical Cal7 HA proteins. However, the HA antigen yield of the other 7:1 virus, which possessed glutamine at position 223 was 60% of that of NIIDRG-7. In addition, a novel 6:2 virus possessing Cal7 HA and the NA of A/Wisconsin/10/98 (a triple reassortant swine-like H1N1 virus), produced 107% of the HA yield of NIIDRG-7. In this study, we showed that the balance between HA and NA in the influenza A(H1N1)pdm09 virus affects its protein and antigen yield.

収録刊行物

• Japanese Journal of Infectious Diseases

  Japanese Journal of Infectious Diseases 66 (1), 65-68, 2013

  国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会

キーワード

• Infectious Diseases
• Microbiology (medical)
• General Medicine

詳細情報

• CRID: 1390001206241091584
• NII論文ID: 130003381645
• NII書誌ID: AA1132885X
• DOI: 10.7883/yoken.66.65
• ISSN: 18842836
• ISSN: 13446304
• NDL書誌ID: 024217327
• PubMed: 23429089
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024217327
• Web Site: https://search.jamas.or.jp/link/ui/2014000393
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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