Hydrogen Peroxide Helps in the Identification of Monophenols as Possible Substrates of Tyrosinase

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  • GARCÍA-MOLINA Mary of the Sea
    GENZ: Investigation Group of Enzimology, Department of Biochemistry and Molecular Biology A. Faculty of Biology, Regional Campus of International Excellence ``Campus Mare Nostrum'', University of Murcia
  • MUÑOZ-MUÑOZ Joseph Luis
    GENZ: Investigation Group of Enzimology, Department of Biochemistry and Molecular Biology A. Faculty of Biology, Regional Campus of International Excellence ``Campus Mare Nostrum'', University of Murcia
  • BERNA Joseph
    Group of Synthetic Organic Chemistry, Department of Organic Chemistry, Faculty of Chemistry, Regional Campus of International Excellence ``Campus Mare Nostrum'', University of Murcia
  • RODRÍGUEZ-LÓPEZ Joseph Neptune
    GENZ: Investigation Group of Enzimology, Department of Biochemistry and Molecular Biology A. Faculty of Biology, Regional Campus of International Excellence ``Campus Mare Nostrum'', University of Murcia
  • VARÓN Ramón
    Department of Physical Chemistry, Upper Polytechnic School, University of Castilla la Mancha, Spain Avenue s/n, University Campus
  • GARCÍA-CÁNOVAS Francis
    GENZ: Investigation Group of Enzimology, Department of Biochemistry and Molecular Biology A. Faculty of Biology, Regional Campus of International Excellence ``Campus Mare Nostrum'', University of Murcia

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Tyrosinase exists in three forms in the catalytic cycle depending on the oxidation state of the copper: met- (Em), oxy- (Eox), and deoxy- (Ed). When O-quinones, products of the enzymatic reaction, evolve chemically to generate an O-diphenol in the reaction medium, the enzyme acts on a monophenol with O-diphenol as reductant, converting Em to Ed. The binding of Ed to molecular oxygen gives Eox, which is active on monophenols, but when the O-quinone product does not generate O-diphenol through chemical evolution, the monophenol does not act as an enzyme substrate. The fact that Eox can be formed from Em with hydrogen peroxide can be used to help identify whether a monophenol is a substrate of tyrosinase. The results obtained in this study confirm that compounds previously described as inhibitors of the enzyme are true substrates of it.

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