Hypoxic Preconditioning Increases Survival of Cardiac Progenitor Cells via the Pim-1 Kinase-Mediated Anti-Apoptotic Effect
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- Hu Shengda
- Department of Cardiology, Zhongda Hospital, Medical School, Southeast University
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- Yan Gaoliang
- Department of Cardiology, Zhongda Hospital, Medical School, Southeast University
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- Xu Hongzeng
- Department of Cardiology, Zhongda Hospital, Medical School, Southeast University
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- He Wei
- Department of Cardiothoracic Surgery, Zhongda Hospital, Medical School, Southeast University
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- Liu Zhiyong
- Department of Cardiothoracic Surgery, Zhongda Hospital, Medical School, Southeast University
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- Ma Genshan
- Department of Cardiology, Zhongda Hospital, Medical School, Southeast University
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Abstract
Background: Stem cells transplanted to the ischemic myocardium usually encounter massive cell death within a few days after transplantation, and hypoxic preconditioning (HPC) is currently used as a strategy to prepare stem cells for increased survival and engraftment in the heart. The purpose of this study is to determine whether Pim-1 kinase mediates any beneficial effects of HPC for human cardiac progenitor cells (CPCs). Methods and Results: Human CPCs were isolated from an adult heart auricle and were purified by magnetic-activated cell sorting using c-kit magnetic beads; they were hypoxic preconditioned for 6h. Both Pim-1 and p-Akt were determined. CPCs were assigned to one of the following groups: (1) control (without HPC); (2) HPC; or (3) HPC+I (Pim-1 inhibitor). HPC can promote the survival of CPCs. HPC enhances the expression of Pim-1 kinase in a time-dependent manner, which causes a reduction of proapoptotic elements (cytochrome c and cleaved caspase-3) and the preservation/modulation of important components of the mitochondria (Bcl-2, Bcl-XL and p-Bad), and attenuates mitochondrial damages. All of these protective effects were blocked by a Pim-1 inhibitor. Conclusions: Pim-1 plays a pivotal role in the protective effect of HPC for CPCs, and the promotion of the expression of Pim-1 in CPCs can as serve part of molecular therapeutic interventional strategies in the treatment of cardiomyopathy damage by blunting CPC death. (Circ J 2014; 78: 724–731)<br>
Journal
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- Circulation Journal
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Circulation Journal 78 (3), 724-731, 2014
The Japanese Circulation Society
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Details 詳細情報について
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- CRID
- 1390001205106628352
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- NII Article ID
- 130003382258
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- NII Book ID
- AA11591968
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- COI
- 1:STN:280:DC%2BC2czjtFCitw%3D%3D
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- ISSN
- 13474820
- 13469843
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- NDL BIB ID
- 025294121
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- PubMed
- 24401608
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed