Developmental Changes in Excitation–Contraction Mechanisms of the Mouse Ventricular Myocardium as Revealed by Functional and Confocal Imaging Analyses
-
- Hamaguchi Shogo
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Japan
-
- Kawakami Yuko
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Japan
-
- Honda Yoriko
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Japan
-
- Nemoto Kana
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Japan
-
- Sano Ayaka
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Japan
-
- Namekata Iyuki
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Japan
-
- Tanaka Hikaru
- Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Japan
この論文をさがす
抄録
Developmental changes in excitation–contraction mechanisms were examined in the ventricular myocardium from fetal, neonatal, and 1-, 2-, and 4-week-old mice. In isolated tissue, the negative inotropic effect of nifedipine decreased, while that of ryanodine increased with age. Action potential duration decreased with age, especially during the late fetal period. In ventricular cardiomyocytes, fluorescence imaging revealed that the sarcoplasmic reticulum increases progressively during pre- and postnatal development. t-Tubules were absent in the fetus and neonate, were observed only in the subsarcolemmal region at 1 week after birth, and were present throughout the cytoplasm at 2 and 4 weeks after birth. The amplitude of Ca2+ transients, as well as its ryanodine-sensitive component, increased with age. In the neonate and 1-week-old mice, Ca2+ at the cell center showed slower rise than the subsarcolemmal region, but in 2- and 4-week-old mice, Ca2+ increased simultaneously across the entire width of the cell. These results suggest that in the mouse ventricular myocardium, the shortening of the action potential during the late fetal period and the development of t-tubule–sarcoplasmic reticulum coupling during the second postnatal week largely contribute to the developmental increase in the dependence of contraction on sarcoplasmic reticulum function.
収録刊行物
-
- Journal of Pharmacological Sciences
-
Journal of Pharmacological Sciences 123 (2), 167-175, 2013
公益社団法人 日本薬理学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282680157368832
-
- NII論文ID
- 130003382594
-
- NII書誌ID
- AA11806667
-
- COI
- 1:CAS:528:DC%2BC3sXhslertrrL
-
- ISSN
- 13478648
- 13478613
-
- NDL書誌ID
- 024954895
-
- PubMed
- 24096881
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可