The function of DrPax1b gene in the embryonic development of zebrafish
-
- Liu Xin
- State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University
-
- Wang Hua
- State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University
-
- Li Guang
- State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University
-
- Huang Hui-Zhe
- State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University
-
- Wang Yi-Quan
- State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University
書誌事項
- タイトル別名
-
- The function of <i>DrPax1b</i> gene in the embryonic development of zebrafish
この論文をさがす
抄録
Vertebrate Pax1 gene is a member of Pax gene family and encodes a transcription factor associated with crucial roles in the development of pharyngeal pouch, scletrotome and limb bud. In zebrafish, the genome contains two Pax1 paralogs, DrPax1a and DrPax1b, which share high sequence similarity with other Pax1 genes. To elucidate the function of zebrafish DrPax1b gene, we first examined the gene expression pattern and found that it was mainly expressed in the endodermal pharyngeal pouch, caudal somites, notochord, and fin bud. Then, we performed knockdown experiments using antisense morpholino oligonucleotides, which lead to the defects in the vertebral column, tail, pharyngeal skeleton, and pectoral fin. Additionally, we also found that the mouse MmPax1 mRNA, but not the amphioxus AmphiPax1/9 mRNA, could rescue the MO-induced defects. Furthermore, sequence alignment revealed that the N-terminal region of vertebrate Pax1 and amphioxus Pax1/9 were highly conserved, whereas their C-terminal regions were relatively divergent. However, the chimeric Am(N)Dr(C)Pax1, Mm(N)Dr(C)Pax1 and Dr(N)Mm(C)Pax1 mRNA could partially rescue the defects, while the Dr(N)Am(C)Pax1 mRNA could not. In conclusion, our data demonstrate a conserved function of DrPax1b in the development of the vertebral column, pectoral fin and pharyngeal skeleton formation in zebrafish and also provide critical insight into the functional evolution of Pax1 gene by changing its C-terminal sequence.
収録刊行物
-
- Genes & Genetic Systems
-
Genes & Genetic Systems 88 (4), 261-269, 2013
日本遺伝学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205473954432
-
- NII論文ID
- 130003390807
-
- NII書誌ID
- AA11077421
-
- COI
- 1:STN:280:DC%2BC2czptFChtA%3D%3D
-
- ISSN
- 18805779
- 13417568
-
- NDL書誌ID
- 025152591
-
- PubMed
- 24463529
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可