Cellular Imaging of Cadmium in Resin Sections of Arbuscular Mycorrhizas Using Synchrotron Micro X-ray Fluorescence

• Nayuki Keiichiro

  Graduate School of Integrated Arts and Sciences, Hiroshima University Graduate School of Agriculture, Shinshu University

• Chen Baodong

  Nasu Research Station, NARO Institute of Livestock and Grassland Science

• Ohtomo Ryo

  Nasu Research Station, NARO Institute of Livestock and Grassland Science

• Kuga Yukari

  Graduate School of Integrated Arts and Sciences, Hiroshima University

抄録

Arbuscular mycorrhizal (AM) fungi function as extended roots and take an active part in plant acquisition of nutrients and also soil pollutants, such as heavy metals. The objective of this study was to establish a method to observe the localization of cadmium (Cd) Kα at subcellular levels using X-ray fluorescence (XRF) imaging with a synchrotron irradiation microbeam in resin-embedded sections of mycorrhizas. To evaluate the methodology, distributions of Cd in high-pressure-frozen Lotus japonicus—Rhizophagus irregularis mycorrhizal roots were compared between two treatments; Cd was exposed either to the roots or to the extraradical hyphae. Results showed that, in the latter treatment, Cd was restricted to fungal structures, whereas in the former, Cd was detected in cell walls of the two organisms. Plunge-frozen extraradical mycelium of Gigaspora margarita exposed to Cd showed high signals of Cd in the cell walls and vacuoles, and low in the cytoplasm. With selective staining and elemental mapping by electron-dispersive X-ray spectrometry (EDS), a positive correlation between distributions of Cd and P was revealed in the vacuole, which suggested polyP as a counter ion of Cd. These results indicated that there was no Cd relocation in rapidly frozen resin-embedded materials, therefore supporting the usefulness of this methodology.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 29 (1), 60-66, 2014

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• arbuscular mycorrhiza
• high-pressure freezing technique
• synchrotron micro XRF
• polyphosphate
• EDS-SEM

詳細情報

• CRID: 1390282679321882624
• NII論文ID: 40020028959
• NII論文ID: 130003390906
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me13093
• COI: 1:STN:280:DC%2BC2cvisVegsw%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 025368574
• PubMed: 24499974
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025368574
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/29/1/29_ME13093/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可