Adipose-Derived Stem Cells Stimulate Reendothelialization in Stented Rat Abdominal Aorta

• Sato Tomohiko

  Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• Takahashi Masao

  Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• Fujita Daishi

  Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• Oba Shigeyoshi

  Department of Internal Medicine, Faculty of Medicine, University of Tokyo

• Nishimatsu Hiroaki

  Department of Urology, Faculty of Medicine, University of Tokyo

• Nagano Tetsuo

  Graduate School of Pharmaceutical Sciences, University of Tokyo

• Suzuki Etsu

  St. Marianna University School of Medicine

抄録

Background: Although drug-eluting stents (DES) have been widely used for the treatment of coronary artery disease, they potentially increase the risk of late thrombosis. It is, therefore, desirable to establish a strategy to stimulate reendothelialization. Endothelial injury models have been widely used to analyze the mechanisms of coronary restenosis. However, animal models deployed with coronary stents in the blood vessels are necessary to accurately analyze the mechanisms of coronary restenosis and late thrombosis because persistent inflammation occurs around the coronary stents. Methods and Results: Coronary stents were implanted into rat abdominal aorta and adipose tissue-derived stem cells (ASC) were administered from the adventitial side. Reendothelialization was then visualized by Evans blue staining, and neointimal formation was analyzed histologically. ASC significantly stimulated reendothelialization and inhibited neointimal formation in bare metal stents (BMS)-implanted aorta. In addition, ASC promoted reendothelialization in DES-implanted aorta; however, the effects were weaker than in BMS-implanted aorta. Among the cytokines that ASC produce, adrenomedullin (AM) significantly stimulated reendothelialization and inhibited neointimal formation in BMS-implanted aorta, when an adenovirus expressing AM was administered from the adventitial side. Conclusions: These results suggest that ASC produce several cytokines that stimulate reendothelialization and inhibit neointimal formation in stent-deployed vessels, and that AM could mediate these effects.  (Circ J 2014; 78: 1762–1769)<br>

収録刊行物

• Circulation Journal

  Circulation Journal 78 (7), 1762-1769, 2014

  一般社団法人 日本循環器学会

キーワード

• Adipose tissue-derived stem cells
• Adrenomedullin
• Coronary stent
• Neointimal formation
• Reendothelialization

詳細情報

• CRID: 1390001205106369408
• NII論文ID: 130003391139
• NII書誌ID: AA11591968
• DOI: 10.1253/circj.cj-13-1579
• COI: 1:STN:280:DC%2BC2cnmtFaiuw%3D%3D
• ISSN: 13474820
• ISSN: 13469843
• NDL書誌ID: 025533896
• PubMed: 24758766
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025533896
• Web Site: https://www.jstage.jst.go.jp/article/circj/78/7/78_CJ-13-1579/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015132878
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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