Nested PCR Detection and Phylogenetic Analysis of Babesia bovis and Babesia bigemina in Cattle from Peri-Urban Localities in Gauteng Province, South Africa
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- MTSHALI Phillip Senzo
- Veterinary Parasitology Unit, Research and Scientific Services Department, National Zoological Gardens of South Africa, Pretoria, South Africa
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- TSOTETSI Ana Mbokeleng
- Agricultural Research Council-Onderstepoort Veterinary Institute, Onderstepoort, South Africa Parasitology Research Program, Department of Zoology and Entomology, University of the Free State, Qwaqwa Campus, Phuthaditjhaba, South Africa
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- THEKISOE Matlhahane Molifi Oriel
- Parasitology Research Program, Department of Zoology and Entomology, University of the Free State, Qwaqwa Campus, Phuthaditjhaba, South Africa
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- MTSHALI Moses Sibusiso
- Veterinary Parasitology Unit, Research and Scientific Services Department, National Zoological Gardens of South Africa, Pretoria, South Africa Parasitology Research Program, Department of Zoology and Entomology, University of the Free State, Qwaqwa Campus, Phuthaditjhaba, South Africa
書誌事項
- タイトル別名
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- Nested PCR Detection and Phylogenetic Analysis of <i>Babesia bovis</i> and <i>Babesia bigemina</i> in Cattle from Peri-Urban Localities in Gauteng Province, South Africa
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Babesia bovis and Babesia bigemina are tick-borne hemoparasites causing babesiosis in cattle worldwide. This study was aimed at providing information about the occurrence and geographical distribution of B. bovis and B. bigemina species in cattle from Gauteng province, South Africa. A total of 268 blood samples collected from apparently healthy animals in 14 different peri-urban localities were tested using previously established nested PCR assays for the detection of B. bovis and B. bigemina species-specific genes encoding rhoptry-associated protein 1 (RAP-1) and SpeI-AvaI restriction fragment, respectively. Nested PCR assays revealed that the overall prevalence was 35.5% (95% confidence interval [CI]=± 5.73) and 76.1% (95% CI=± 5.11) for B. bovis and B. bigemina, respectively. PCR results were corroborated by sequencing amplicons of randomly selected samples. The neighbor-joining trees were constructed to study the phylogenetic relationship between B. bovis and B. bigemina sequences of randomly selected isolates. Analysis of phylogram inferred with B. bovis RAP-1 sequences indicated a close relationship between our isolates and GenBank strains. On the other hand, a tree constructed with B. bigemina gp45 sequences revealed a high degree of polymorphism among the B. bigemina isolates investigated in this study. Taken together, the results presented in this work indicate the high incidence of Babesia parasites in cattle from previously uncharacterised peri-urban areas of the Gauteng province. These findings suggest that effective preventative and control measures are essential to curtail the spread of Babesia infections among cattle populations in Gauteng.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 76 (1), 145-150, 2014
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206429370752
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- NII論文ID
- 130003391245
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC2c%2FhtFOkug%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 025173654
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- PubMed
- 24065081
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可