Immunology : Immunogenicity of a Recombinant Mycobacterium smegmatis Vaccine Expressing the Fusion Protein CMX in Cattle from Goias state, Brazil

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  • ALVES DA SILVA Duanne
    Tropical Pathology and Public Health Institute, Federal University of Goiás, Rua 235 esquina com 1<sup>a</sup> Avenida, Setor Universitário, Goiânia, Goiás, CEP 74605–050, Brazil Animal Science Graduate Program, Zootechny and Veterinary School, Federal University of Goiás, Rua Nova Veneza, No. 131 Campus Samambaia, Goiânia, Goiás, Brazil. CEP: 74001-970
  • CAVALCANTI Marcos Antônio Rocha
    Tropical Pathology and Public Health Institute, Federal University of Goiás, Rua 235 esquina com 1<sup>a</sup> Avenida, Setor Universitário, Goiânia, Goiás, CEP 74605–050, Brazil Animal Science Graduate Program, Zootechny and Veterinary School, Federal University of Goiás, Rua Nova Veneza, No. 131 Campus Samambaia, Goiânia, Goiás, Brazil. CEP: 74001-970 Goiano Federal Institute, Campus Urutaí, Km 2,5 Rua Geraldo Silva Nascimento, Urutaí, Goiás, CEP 75790–000, Brazil
  • MUNIZ DE OLIVEIRA Fábio
    Tropical Pathology and Public Health Institute, Federal University of Goiás, Rua 235 esquina com 1<sup>a</sup> Avenida, Setor Universitário, Goiânia, Goiás, CEP 74605–050, Brazil
  • TRENTINI Monalisa Martins
    Tropical Pathology and Public Health Institute, Federal University of Goiás, Rua 235 esquina com 1<sup>a</sup> Avenida, Setor Universitário, Goiânia, Goiás, CEP 74605–050, Brazil
  • JUNQUEIRA-KIPNIS Ana Paula
    Tropical Pathology and Public Health Institute, Federal University of Goiás, Rua 235 esquina com 1<sup>a</sup> Avenida, Setor Universitário, Goiânia, Goiás, CEP 74605–050, Brazil
  • KIPNIS André
    Tropical Pathology and Public Health Institute, Federal University of Goiás, Rua 235 esquina com 1<sup>a</sup> Avenida, Setor Universitário, Goiânia, Goiás, CEP 74605–050, Brazil

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  • Immunogenicity of a Recombinant <i>Mycobacterium smegmatis</i> Vaccine Expressing the Fusion Protein CMX in Cattle from Goiás State, Brazil

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This study aimed to evaluate the immunogenicity of a recombinant Mycobacterium smegmatis vaccine expressing the CMX fusion protein composed of immunodominant epitopes Ag85C, MPT51 and HspX of Mycobacterium tuberculosis, which are important mycobacteria virulence factors. A group of Nelore heifers that were 10 to 12 months of age and negative for the tuberculin skin test (TST) were immunized with four doses of the recombinant vaccine mc2-CMX (M. smegmatis-Ag85C-MPT51-HspX) during a period of one year. Before each immunization, blood was collected to obtain sera for antibody analysis. Serological analysis demonstrated that mc2-CMX was able to induce a humoral response with increased levels of specific IgG antibodies against CMX, despite minimum antibody levels being detected for individual Ag85C, MPT51 or HspX recombinant antigens. However, there was no significant increase in specific CD4+ IFN-γ-positive T cells. Lymphadenomegaly was observed in superficial cervical lymph nodes adjacent to the site of vaccination among mc2-CMX-vaccinated bovines, and the histopathological analysis demonstrated follicular hyperplasia without inflammatory infiltrate or granuloma formation. Animals remained negative for the TST until the end of the experiments, showing no cross-reactivity with the recombinant vaccine and tuberculin proteins. We discuss the potential of mc2-CMX to induce an immune response in cattle.

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