VP16 fusion efficiently reveals the function of transcriptional repressors in Arabidopsis
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- Fujiwara Sumire
- Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Kigoshi Keiko
- Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Mitsuda Nobutaka
- Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Suzuki Kaoru
- Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- Ohme-Takagi Masaru
- Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Institute for Environmental Science and Technology, Saitama University
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抄録
Proper gene expression regulated by transcription factors is essential for plants to achieve proper growth and development. However, the biological functions of many transcription factors remain largely unknown. Furthermore, although there are transcription factors which possess a plant-specific repression domain(s), their biological functions and whether such transcription factors function as transcriptional repressors are unclear. Thus, aiming for searching clues to understand their functions, we generated transgenic plants in which a putative transcriptional repressor fused with a VP16 viral trans-activation domain was expressed constitutively. Several plants with strong morphological phenotypes such as leaf and flower development defects were isolated from those lines expressing potential transcriptional repressors with unknown functions, giving the clue to reveal the yet-to-be analyzed functions of each protein. Reversal of function of the well-known transcriptional and floral repressor SHORT VEGETATIVE PHASE by VP16 fusion was observed, exemplifying successful functional reversion by this system. Plants constitutively expressing VP16 fused WUSCHEL, which is known to function both as a transcriptional activator and repressor, showed both phenotypes reported in its overexpression and loss-of-function lines. Taken together, our data provide examples showing the efficacy of VP16 fusion to provide helpful information to uncover the unknown functions of potential transcriptional repressors. This technique could also be effective to produce “super plants” which obtained strong and useful traits for application by strongly activating genes which are usually silent.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 31 (2), 123-132, 2014
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204328664320
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- NII論文ID
- 130003391373
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 025551026
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可