Potential Involvement of μ-Opioid Receptor Dysregulation on the Reduced Antinociception of Morphine in the Inflammatory Pain State in Mice
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- Aoki Yuta
- Department of Physiology and Anatomy, Tohoku Pharmaceutical University, Japan
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- Mizoguchi Hirokazu
- Department of Physiology and Anatomy, Tohoku Pharmaceutical University, Japan
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- Watanabe Chizuko
- Department of Physiology and Anatomy, Tohoku Pharmaceutical University, Japan
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- Takeda Kumiko
- Department of Physiology and Anatomy, Tohoku Pharmaceutical University, Japan
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- Sakurada Tsukasa
- First Department of Pharmacology, Daiichi College of Pharmaceutical Sciences, Japan
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- Sakurada Shinobu
- Department of Physiology and Anatomy, Tohoku Pharmaceutical University, Japan
書誌事項
- タイトル別名
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- Potential Involvement of <i>μ</i>-Opioid Receptor Dysregulation on the Reduced Antinociception of Morphine in the Inflammatory Pain State in Mice
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The antinociceptive effect of morphine in the inflammatory pain state was described in the von Frey filament test using the complete Freund’s adjuvant (CFA)-induced mouse inflammatory pain model. After an i.pl. injection of CFA, mechanical allodynia was observed in the ipsilateral paw. The antinociceptive effect of morphine injected s.c. and i.t. against mechanical allodynia was reduced bilaterally at 1 day and 4 days after the CFA pretreatment. The expression level of mRNA for μ-opioid receptors at 1 day after the CFA pretreatment was reduced bilaterally in the lumbar spinal cord and dorsal root ganglion (DRG). In contrast, the protein level of μ-opioid receptors at 1 day after CFA pretreatment was decreased in the ipsilateral side in the DRG but not the lumbar spinal cord. Single or repeated i.t. pretreatment with the protein kinase Cα (PKCα) inhibitor Ro-32-0432 completely restored the reduced morphine antinociception in the contralateral paw but only partially restored it in the ipsilateral paw in the inflammatory pain state. In conclusion, reduced morphine antinociception against mechanical allodynia in the inflammatory pain state is mainly mediated via a decrease in μ-opioid receptors in the ipsilateral side and via the desensitization of μ-opioid receptors in the contralateral side by PKCα-induced phosphorylation.
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 124 (2), 258-266, 2014
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390282680155921536
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- NII論文ID
- 130003391472
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- NII書誌ID
- AA11806667
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- COI
- 1:CAS:528:DC%2BC2cXkt1egt7c%3D
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 025296284
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- PubMed
- 24553404
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可