Lumbar Intervertebral Disc Puncture under C-arm Fluoroscopy: a New Rat Model of Lumbar Intervertebral Disc Degeneration

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  • Li Dapeng
    Department of Orthopaedic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, 212001, P.R. China Department of Orthopaedic Surgery, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, P.R. China
  • Yang Huilin
    Department of Orthopaedic Surgery, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, P.R. China
  • Huang Yonghui
    Department of Orthopaedic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, 212001, P.R. China Department of Orthopaedic Surgery, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, P.R. China
  • Wu Yan
    Department of Orthopaedic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, 212001, P.R. China
  • Sun Taicun
    Department of Orthopaedic Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, 212001, P.R. China
  • Li Xuefeng
    Department of Orthopaedic Surgery, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, P.R. China

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To establish a minimally invasive rat model of lumbar intervertebral disc degeneration (IDD) to better understand the pathophysiology of the human condition. The annulus fibrosus of lumbar level 4–5 (L4-5) and L5-6 discs were punctured by 27-gauge needles using the posterior approach under C-arm fluoroscopic guidance. Magnetic resonance imaging (MRI), histological examination by hematoxylin and eosin (H&E) staining, and reverse transcription polymerase chain reaction (RT-PCR) were performed at baseline and 2, 4, and 8 weeks after disc puncture surgery to determine the degree of degeneration. All sixty discs (thirty rats) were punctured successfully. Only two of thirty rats subjected to the procedure exhibited immediate neurological symptoms. The MRI results indicated a gradual increase in Pfirrmann grade from 4 to 8 weeks post-surgery (P<0.05), and H&E staining demonstrated a parallel increase in histological grade (P<0.05). Expression levels of aggrecan, type II collagen (Col2), and Sox9 mRNAs, which encode disc components, decreased gradually post-surgery. In contrast, mRNA expression of type I collagen (Col1), an indicator of fibrosis, increased (P<0.05). The procedure of annular puncture using a 27-gauge needle under C-arm fluoroscopic guidance had a high success rate. Histological, MRI, and RT-PCR results revealed that the rat model of disc degeneration is a progressive pathological process that is similar to human IDD.

収録刊行物

  • Experimental Animals

    Experimental Animals 63 (2), 227-234, 2014

    公益社団法人 日本実験動物学会

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