Transcriptional and Histological Analyses of the Thymic Developmental Process in the Fetal Pig

• Suzuki Shunichi

  Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan

• Suzuki Misae

  Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan

• Nakai Michiko

  Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan

• Sembon Shoichiro

  Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan

• Fuchimoto Daiichiro

  Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan

• Onishi Akira

  Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan

抄録

The humanized pig model, in which human cells or tissues can be functionally maintained in pigs, can be an invaluable tool for human medical research. Although the recent development of immunodeficient pigs has opened the door for the development of such a model, the efficient engraftment and differentiation of human cells may be difficult to achieve. The transplantation of human cells into fetal pigs, whose immune system is immature, will ameliorate this problem. Therefore, we examined the development of porcine fetal thymus, which is critical for the establishment of the immune system. We first analyzed the levels of mRNA expression of genes that are relevant to the function of thymic epithelial cells or thymocytes in whole thymi from 35 to 85 days of gestation (DG) and at 2 days postpartum (DP) by quantitative RT-PCR. In addition, immunohistochemical analyses of thymic epithelial cells from DG35 to DG55 and DP2 were performed. These analyses showed that the thymic cortex was formed as early as DG35, and thymic medulla gradually developed from DG45 to DG55. These findings suggested that, at least before DG45, the thymus do not differentiate to form fully functional T cells.

収録刊行物

• Experimental Animals

  Experimental Animals 63 (2), 215-225, 2014

  公益社団法人 日本実験動物学会

キーワード

• fetal pig
• gene expression
• immunohistochemistry
• thymus

詳細情報

• CRID: 1390001205044046464
• NII論文ID: 130003391620
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.63.215
• COI: 1:STN:280:DC%2BC2cnntlyjtw%3D%3D
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 025412054
• PubMed: 24770647
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025412054
• Web Site: https://www.jstage.jst.go.jp/article/expanim/63/2/63_13-0068/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014344237
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可