Identification and quantification of amyloid beta-related peptides in human plasma using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
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- KANEKO Naoki
- Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation
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- YAMAMOTO Rie
- Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation
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- SATO Taka-Aki
- Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation Life Science Research Center, Shimadzu Corporation
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- TANAKA Koichi
- Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation
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抄録
Proteolytic processing of the amyloid precursor protein (APP) by β-secretase and γ-secretase leads to the generation and deposition of amyloid β (Aβ) in Alzheimer’s disease (AD). N-terminally or C-terminally truncated Aβ variants have been found in human cerebrospinal fluid and cultured cell media using immunoprecipitation and mass spectrometry. Unfortunately, the profile of plasma Aβ variants has not been revealed due to the difficulty of isolating Aβ from plasma. We present here for the first time studies of Aβ and related peptides in human plasma. Twenty-two Aβ-related peptides including novel peptides truncated before the β-secretase site were detected in human plasma and 20 of the peptides were identified by tandem mass spectrometry. Using an internal standard, we developed a quantitative assay for the Aβ-related peptides and demonstrated plasma dilution linearity and the precision required for their quantitation. The present method should enhance the understanding of APP processing and clearance in AD progression.
収録刊行物
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- Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences
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Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences 90 (3), 104-117, 2014
日本学士院
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詳細情報 詳細情報について
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- CRID
- 1390282679124369152
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- NII論文ID
- 130003392355
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- NII書誌ID
- AA00785485
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- COI
- 1:STN:280:DC%2BC2crjtFaktg%3D%3D
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- ISSN
- 13492896
- 03862208
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- NDL書誌ID
- 025359708
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- PubMed
- 24621957
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可