Inhibitory Effect of Heat-Treated 3-(3^|^prime;,4^|^prime;-dihydroxyphenyl)-L-alanine (DOPA) on ^|^beta;-glucuronidase Activity

• HASHIMOTO Kei

  Department of Applied Biological Chemistry, Utsunomiya University

• KINPARA Mika

  Department of Applied Biological Chemistry, Utsunomiya University

• UDA Yasushi

  Department of Applied Biological Chemistry, Utsunomiya University

書誌事項

タイトル別名

• Inhibitory Effect of Heat-Treated 3-(3′,4′-dihydroxyphenyl)-<small>L</small>-alanine (DOPA) on β-glucuronidase Activity

抄録

β-Glucuronidase may contribute to the development of colon cancer. A reduction in the activity of this enzyme could inhibit the enterohepatic circulation of carcinogens, which is thought to reduce the risk of colon cancer. The purpose of this study was to investigate the effect of heat treatment on the β-glucuronidase inhibition activity of DOPA. Prior to heating, DOPA showed only a weak inhibitory effect on β-glucuronidase. After heating a DOPA solution at 100°C for 10 min, the heat-treated DOPA (hDOPA) exhibited 48.5% inhibition at 10 µM. We performed a kinetic study on β-glucuronidase inhibition by hDOPA using Lineweaver-Burk analysis. The kinetics profile suggests that hDOPA competitively inhibits β-glucuronidase.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 19 (6), 1115-1119, 2013

  公益社団法人 日本食品科学工学会

キーワード

• 3-(3′,4′-dihydroxyphenyl)-<small>L</small>-alanine (DOPA)
• β-glucuronidase
• microbiota

詳細情報

• CRID: 1390282679435298432
• NII論文ID: 130003392405
• DOI: 10.3136/fstr.19.1115
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可