Characterization of the palI Gene of Aspergillus oryzae

• Sano Motoaki

  Genome Biotechnology Laboratory, Kanazawa Institute of Technology

• Kobayashi Akiko

  Genome Biotechnology Laboratory, Kanazawa Institute of Technology

• Ohashi Shinichi

  Genome Biotechnology Laboratory, Kanazawa Institute of Technology

書誌事項

タイトル別名

• Characterization of the <i>palI</i> Gene of <i>Aspergillus oryzae</i>

抄録

The palI gene promotes plasma membrane localization of PalH in Aspergillus nidulans. We analyzed the role of palI in the ambient pH signal transduction pathway of A. oryzae by disrupting the palI gene. Polymerase chain reaction and Southern hybridization analyses indicated that homologous recombination occurred at the resident palI locus. palI disruption resulted in significantly decreased pacC expression and alkaline protease activity. Based on these results, we believe that palI plays an important role in the ambient pH signal transduction pathway of A. oryzae. While the palI-disrupted strain showed some growth on plates at alkaline pH, the palH-disrupted strain showed no growth. Thus, the results indicate that palI plays an important but not essential role in the pH signal transduction pathway of A. oryzae.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 20 (1), 155-159, 2014

  公益社団法人 日本食品科学工学会

キーワード

• <i>Aspergillus oryzae</i>
• <i>palI</i>
• <i>pacC</i>
• alkaline protease (<i>Alp</i>)
• real-time polymerase chain reaction (PCR)

詳細情報

• CRID: 1390001204459189376
• NII論文ID: 130003392432
• DOI: 10.3136/fstr.20.155
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可