腱板骨付着部変性における酸化ストレスの寄与

DOI

書誌事項

タイトル別名
  • Contribution of oxidative stress on degeneration of rotator cuff enthesis

この論文をさがす

抄録

Background: Rotator cuff degeneration is one of the multiple factors that lead to rotator cuff tear, but the mechanism of degeneration still remains unclear. We have reported that antioxidant enzyme, Sod1, regulates intracellular redox balance and Sod1 deficiency induced musculoskeletal aging, such as bone fragility and skeletal muscle atrophy. In this study, we analyzed supraspinatus tendon insertions to clarify the links between oxidative stress and rotator cuff degeneration.<BR>Methods: Supraspinatus tendon and humeral head were isolated and fixed to prepare histological sections from wild-type (WT) and Sod1-deficient (Sod1-l-) male mice (N = 4) at 20 weeks of age. Haematoxylin-eosin staining was performed to assess the overall histological structure. To investigate structure the of collagen fibers, we examined spatially aligned collagen fibers using a polarizing microscope and the amount of collagens using immunohistochemical staining. To analyze mechanical properties, we measured tissue acoustic properties using scanning acoustic microscopy (SAM).<BR>Results: Sod1-l- mice showed abnormality of four-layered structure and tidemark at the enthesis. Sod1deficiency also decreased the area of brightly diffracted light and reduced the amount of type 1 collagen, indicating collagen down-regulation. SAM analysis revealed that sound speed and attenuation were increased in non-mineralized fibrocartilage of Sod1-l- mice.<BR>Conclusion: Sod1 insufficiency induced cartilage degeneration associated with impaired elasticity in supraspinatus tendon enthesis, recapitulating the human rotator cuff degeneration. These results suggested that oxidative stress may cause rotator cuff tear due to degeneration.

収録刊行物

  • 肩関節

    肩関節 37 (2), 825-828, 2013

    日本肩関節学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ