Particle Monitoring and Effectiveness of Large-Scale Cleaning in a Bone Marrow Transplantation Unit

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  • KIYOSUKE Makiko
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital Center for the Study of Global Infection, Kyushu University Hospital
  • MOROKUMA Yuiko
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital Center for the Study of Global Infection, Kyushu University Hospital
  • KADOWAKI Masako
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital Center for the Study of Global Infection, Kyushu University Hospital Department of Clinical Immunology and Rheumatology/Infectious Disease, Kyushu University Hospital
  • KIBE Yasushi
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
  • YAMASHITA Yuka
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
  • YAMASAKI Mika
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
  • YAMADA Takaaki
    Center for the Study of Global Infection, Kyushu University Hospital
  • YASUNAGA Sachie
    Center for the Study of Global Infection, Kyushu University Hospital
  • GONDO Tae
    Center for the Study of Global Infection, Kyushu University Hospital
  • TOYODA Kazuhiro
    Center for the Study of Global Infection, Kyushu University Hospital Department of General Internal Medicine, Kyushu University Hospital
  • SHIMONO Nobuyuki
    Center for the Study of Global Infection, Kyushu University Hospital Department of Clinical Immunology and Rheumatology/Infectious Disease, Kyushu University Hospital
  • HAYASHI Jun
    Center for the Study of Global Infection, Kyushu University Hospital Department of General Internal Medicine, Kyushu University Hospital

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Other Title
  • 無菌病棟における大規模清掃時の環境汚染状況とその評価
  • ムキン ビョウトウ ニ オケル ダイキボ セイソウジ ノ カンキョウ オセン ジョウキョウ ト ソノ ヒョウカ

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Abstract

  The department of hematology/oncology in our hospital has a bone marrow transplantation (BMT) unit with 31 cleanrooms and associated clean areas. Air cleanliness of the whole unit is maintained at class 7 based on ISO 14644–1, which is equivalent to class 10,000 based on the US Federal Standard 209E. The floor has not only private rooms but also a staff station, lounge, laundry room, etc.; the same arrangement as a normal ward. There are few facilities where the whole floor is kept at class 7 in Japan. The unit is a closed space and environmental management is important for the floor, so we decided to enforce large-scale cleaning in the same way as the operating rooms and ICU under ICT supervision. However, there are no guidelines about cleaning of wards in Japan. Cleaning presents the problem of environmental pollution during the cleaning, and elapsed time before patients can return to their rooms after the cleaning. To evaluate actual contamination status during and after cleaning, the number of microparticles were measured using a particle counter and the colonies counted on the agar plates. The number of microparticles was increased the most after drying of a corridor with a blower after waxing and after moving several beds from the cleanroom to a corridor. The quantity of microparticles stabilized at less than two columns at approximately 30 minutes after cleaning. Multiple colonies of bacteria, yeasts, and fungi were recovered from several samples during the cleaning, but only a few colonies were recovered from samples after cleaning. Most colonies were identified as environmental microorganisms. Therefore, patients should not enter a cleanroom after completion of cleaning for 30–60 minutes. If several cleanrooms are cleaned at the same time, a barrier should be positioned in the corridor to prevent contamination of other areas.<br>

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