Rapid Detection of Microbes in the Dialysis Solution by the Microcolony Fluorescence Staining Method (Millflex Quantum)
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- OSONO EIICHI
- Quality Control Section Quality Assurance Section, Koshigaya Oobukuro Clinic Microbiology and Immunology, Nippon Medical School
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- KOBAYASHI EIKO
- Field Marketing, Merck Millipore, Merck Ltd. Japan KK
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- INOUE YUKI
- Quality Control Section
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- HONDA KAZUMI
- Production Control Section
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- KUMAGAI TAKUYA
- Production Control Section
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- NEGISHI HIDEKI
- Production Control Section
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- OKAMATSU KENTARO
- Quality Assurance Section, Koshigaya Oobukuro Clinic
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- ICHIMURA KYOKO
- Quality Assurance Section, Koshigaya Oobukuro Clinic
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- KAMANO CHISAKO
- Quality Assurance Section, Koshigaya Oobukuro Clinic
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- SUZUKI FUMI
- Field Marketing, Merck Millipore, Merck Ltd. Japan KK
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- NOROSE YOSHIHIKO
- Microbiology and Immunology, Nippon Medical School
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- TAKAHASHI MEGUMI
- Microbiology and Immunology, Nippon Medical School
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- TAKAKU SHUN
- Microbiology and Immunology, Nippon Medical School
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- FUJIOKA NORIAKI
- Okachimachi Jin Clinic
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- HAYAMA NAOAKI
- Saitama Tsukinomori Clinic
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- TAKIZAWA HIDEAKI
- Institute of Social Medicine, Tokyo University of Pharmaceutical and Life Science
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抄録
A chemiluminescence system, Milliflex Quantum (MFQ), to detect microcolonies, has been used in the pharmaceutical field. In this study, we investigated aquatic bacteria in hemodialysis solutions sampled from bioburden areas in 4 dialysis faculties. Using MFQ, microcolonies could be detected after a short incubation period. The colony count detected with MFQ after a 48-hour incubation was 92% ± 39%, compared to that after the conventionally used 7-14-day incubation period； in addition, the results also showed a linear correlation. Moreover, MFQ-based analysis allowed the visualization of damaged cells and of the high density due to the excessive amount of bacteria. These results suggested that MFQ had adequate sensitivity to detect microbacteria in dialysis solutions, and it was useful for validating the conditions of conventional culture methods.
収録刊行物
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- Ｂｉｏｃｏｎｔｒｏｌ Ｓｃｉｅｎｃｅ
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Ｂｉｏｃｏｎｔｒｏｌ Ｓｃｉｅｎｃｅ 19 (1), 57-60, 2014
日本防菌防黴学会
