The inhibitory effect of shakuyakukanzoto on K^+ current in H9c2 cells
-
- SUGANAMI AZUSA
- Department of Anesthesiology, Fukushima Medical University
-
- SAKAMOTO KAZUHO
- Department of Pharmacology, Fukushima Medical University
-
- ONO TOMOYUKI
- Department of Pharmacology, Fukushima Medical University
-
- WATANABE HIROTAKE
- Department of Pharmacology, Fukushima Medical University
-
- HIJIOKA NAHOKO
- Department of Pharmacology, Fukushima Medical University
-
- MURAKAWA MASAHIRO
- Department of Anesthesiology, Fukushima Medical University
-
- KIMURA JUNKO
- Department of Pharmacology, Fukushima Medical University
書誌事項
- タイトル別名
-
- THE INHIBITORY EFFECT OF SHAKUYAKUKANZOTO ON K<sup>+</sup> CURRENT IN H9c2 CELLS
この論文をさがす
抄録
Shakuyakukanzoto (shao-yao-gan-cao-tang) is a commonly used Chinese traditional herbal medicine for the treatment of acute pain with muscle cramp. However, its mechanism of action is unclear. We previously reported that a low concentration of Kanzo (licorice) and isoliquiritigenin, a component of licorice, inhibited the potassium (K+) current in H9c2 cells. Therefore, in the present study, we examined the effects of Shakuyakukanzoto, Shakuyaku or Kanzo on the K+ current (IKur) in H9c2 cells. Shakuyakukanzoto inhibited IKur in a concentration-dependent manner. The half-maximal concentration of Shakuyakukanzoto was approximately 1.3 mg/mL and the Hill coefficient was 1.2. The order of potency of inhibiting IKur was Kanzo>Shakuyakukanzoto>Shakuyaku. Glycyrrhizin, a major component of licorice, had no inhibitory effect on IKur. A small interfering RNA experiment indicated that IKur was most likely to be Kv2.1 in H9c2 cells. Our results suggest that Shakuyakukanzoto may normalize intracellular and extracellular K+ balance by inhibiting IKur and reducing K+ efflux, while the Na+-K+ pump promotes K+ influx into myofibers. Consequently, excess K+ may be reduced from external space of myofibers. This may be a part of the Shakuyakukanzoto mechanism for improving muscle pain.
収録刊行物
-
- 福島医学会
-
福島医学会 60 (1), 22-30, 2014
福島医学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206305211904
-
- NII論文ID
- 130003396126
-
- NII書誌ID
- AA0065246X
-
- COI
- 1:STN:280:DC%2BC2crnsl2rsQ%3D%3D
-
- ISSN
- 21854610
- 00162590
-
- PubMed
- 24670676
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- IRDB
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可