Laser Desorption/Ionization Mass Spectrometry (LDI-MS) of Lipids with Iron Oxide Nanoparticle-Coated Targets

• Kusano Maiko

  Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation

• Kawabata Shin-ichirou

  Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation

• Tamura Yusuke

  Dai Nippon Toryo Co., Ltd.

• Mizoguchi Daigou

  Dai Nippon Toryo Co., Ltd.

• Murouchi Masato

  Dai Nippon Toryo Co., Ltd.

• Kawasaki Hideya

  Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials, and Bioengineering, Kansai University

• Arakawa Ryuichi

  Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials, and Bioengineering, Kansai University

• Tanaka Koichi

  Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation

抄録

Iron oxide nanoparticle (NP)-coated target plates were employed for the direct detection and analysis of low molecular weight lipids by laser desorption/ionization (LDI) mass spectrometry (MS). We have demonstrated that the use of the iron oxide NP-coated target provides a simple, direct, and rapid detection method for lipid standards and epidermal surface lipids without any cumbersome sample pretreatment as well as mass spectra that are free of background matrix peaks. Lipid standards (1-stearoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-sn-glycerol, 1-palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol, 1,2-distearoyl-sn-glycero-3-phosphocholine) were detected as either protonated or cationated species. Clean MS/MS spectra for each lipid were also successfully obtained. Pre-MS surface cleaning of the target plates with UV-ozone treatment successfully removed organic contaminants that would interfere with the mass spectra especially in the low molecular weight region. Preliminary application of the presented target plate to the detection of endogenous lipids in latent fingerprints showed promising results and for potential use in the visualization and chemical composition determination of latent fingerprints by nanoparticle assistance.

収録刊行物

• Mass Spectrometry

  Mass Spectrometry 3 (1), A0026-A0026, 2014

  一般社団法人　日本質量分析学会

キーワード

• iron oxide nanoparticle
• laser desorption/ionization
• mass spectrometry
• lipids
• MALDI
• SALDI

詳細情報

• CRID: 1390001205416464256
• NII論文ID: 130003398583
• DOI: 10.5702/massspectrometry.a0026
• COI: 1:STN:280:DC%2BC2cjlvFCrtw%3D%3D
• ISSN: 21865116
• ISSN: 2187137X
• Web Site: https://www.jstage.jst.go.jp/article/massspectrometry/3/1/3_A0026/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可