DNA from bacteria, but not from vertebrates, induces interferon, activates natural killer cells and inhibits tumor growth
-
- YAMAMOTO Saburo
- National Institute of Health
-
- YAMAMOTO Toshiko
- National Institute of Health Japanese Foundation for AIDS Prevention
-
- SHIMADA Shizuo
- Mitsui Pharmaceuticals Inc., Institute of Biological Science
-
- KURAMOTO Etsuro
- Mitsui Pharmaceuticals Inc., Institute of Biological Science
-
- YANO Osamu
- Mitsui Pharmaceuticals Inc., Institute of Biological Science
-
- KATAOKA Tetsuro
- National Institute of Health
-
- TOKUNAGA Tohru
- National Institute of Health
書誌事項
- タイトル別名
-
- DNA from Bacteria, but Not from Vertebrates, Induces Interferons, Activates Natural Killer Cells and Inhibits Tumor Growth
抄録
The nucleic acid fraction from cells of 6 species of bacterium and 2 kinds of vertebrate, calf and salmon, was extracted and purified by the same procedures as described previously. When the spleen cells from BALB/c mice were incubated with the nucleic acid fraction from either of the bacteria, natural killer (NK) activity of the cells was remarkably elevated and the cells produced factors to activate macrophages and to inhibit viral growth. It was shown that the factor to activate macrophages was interferon (IFN)-gamma and that to inhibit viral growth was IFN-alpha/beta. On the other hand, the nucleic acid fraction from either of the vertebrate cells did not show such activities. Pretreatment of the bacterial nucleic acid fraction with DNase, but not with RNase, abrogated completely the biological activities. The activities of the bacterial nucleic acid were not influenced by the presence of polymyxin B, an inhibitor of lipopolysaccharide (LPS), and the spleen cells from not only BALB/c mice but also LPS-insensitive C3H/HeJ mice were activated, indicating that the activities of the fraction were not ascribed to LPS contaminated possibly into the fraction, but to DNA itself. Intralesional injection with the bacterial DNA fraction caused regression of mouse IMC tumors, but the injection with the vertebrate DNA fraction did not. These findings prompted us to examine the biological activities of DNA samples from a variety of animals and plants, which were provided from other laboratories or purchased from manufacturers. All of the DNA samples from cells of 5 kinds of bacterium, 2 of virus and 4 of invertebrate augmented NK activity and induced IFN, more or less, in mouse spleen calls, while the DNA from 10 kinds of vertebrate, including 3 of fish and 5 of mammal, showed no such activities. The DNA from 2 species of plants, were also inactive. Possible mechanisms to explain the different biological activities of DNA from different cell sources were discussed based on our previous finding that the particular palindromic sequences with a G-C motif (s) are required for induction of IFNs and activation of NK cells with synthetic 30-mer oligonucleotides.
収録刊行物
-
- MICROBIOLOGY and IMMUNOLOGY
-
MICROBIOLOGY and IMMUNOLOGY 36 (9), 983-997, 1992
財団法人 学会誌刊行センター