Inhibitory effect of antibiotic cerulenin on the respiratory burst in phagocytes. II. Inhibition by cerulenin of intracellular calcium mobilization in human neutrophils.
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- NAKATA MUNEHIRO
- The Institute of Medical Science, The University of Tokyo Present address: RIKEN, The Institute of Physical and Chemical Research
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- TOMITA TOSHIO
- The Institute of Medical Science, The University of Tokyo
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- IIZUKA TETSUTARO
- RIKEN, The Institute of Physical and Chemical Research
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- KANEGASAKI SHIRO
- The Institute of Medical Science, The University of Tokyo
書誌事項
- タイトル別名
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- II. INHIBITION BY CERULENIN OF INTRACELLULAR CALCIUM MOBILIZATION IN HUMAN NEUTROPHILS
抄録
Generation of superoxide anion (O2-) and mobilization of intracellular Ca2+ in human neutrophils upon exposure to stimuli such as N-formylmethionylleucylphenylalanine (fMLP) were inhibited by the antibiotic cerulenin, which inhibits fatty acid and cholesterol biosynthesis. The inhibition of O2- generation and Ca2+-mobilization required certain periods of incubation with cerulenin and both abilities of the cells were gradually lost following a similar time-course. In contrast, significant Ca2+-influx from the medium was observed in cerulenin-treated cells as well as untreated cells. The results suggest that an event which coincides with the Ca2+mobilization and not Ca2+ per se is important for the induction of O2- generation in the fMLP-stimulated cells and that this step is blocked in cerulenin-treated cells. Phorbol myristate acetate or synthetic l-oleoyl-2-acetylglycerol were able to bypass the block and induced O2- generation in cerulenin-treated cells.
収録刊行物
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- The Journal of Antibiotics
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The Journal of Antibiotics 42 (7), 1178-1183, 1989
公益財団法人 日本感染症医薬品協会