Properties of Hepatitis Delta Virus Ribozyme, Which Consists of Three RNA Oligomer Strands.

• Sakamoto Taiichi

  Department of Bioengineering, Faculty of Engineering, Yokohama National University

• Tanaka Yoichiro

  Department of Bioengineering, Faculty of Engineering, Yokohama National University

• Kuwabara Tomoko

  Department of Bioengineering, Faculty of Engineering, Yokohama National University

• Kim Mi Hee

  Department of Bioengineering, Faculty of Engineering, Yokohama National University

• Kurihara Yasuyuki

  Department of Bioengineering, Faculty of Engineering, Yokohama National University

• Katahira Masato

  Department of Bioengineering, Faculty of Engineering, Yokohama National University

• Uesugi Seiichi

  Department of Bioengineering, Faculty of Engineering, Yokohama National University

抄録

Properties of a hepatitis delta virus (HDV) RNA ribozyme system, which consists of three RNA oligomer strands (substrate 8-mer ; enzyme 16-mer plus 35-mer) and contains a hybrid sequence of genomic and antigenomic RNA cores, are reported. Effects of Mg²⁺ concentration, divalent metal ion species, pH, and temperature on the cleavage activity were examined. The substrate cleavage activity increased with increasing Mg2+ concentration (0-100 mM). Ca²⁺ and Mn²⁺ ions were the most effective divalent cations and Mg²⁺ was less effective. The cleavage activity increased with increasing pH (5-7.5). The optimum temperature for the cleavage activity was 25-40°C. The Mg²⁺ concentration, pH and temperature dependencies are different from those reported for the single-strand ribozymes (about 90-mer) although the divalent metal ion preference is very similar. Conformational change induced by Mg²⁺ ion titration was monitored by CD. The CD data and the activity-Mg²⁺ concentration data were analyzed by curve-fitting analysis using equations derived for multiple metal ion binding mechanisms. The data can be explained by a model in which three Mg²⁺ ions bind to one ribozyme unit

収録刊行物

• The Journal of Biochemistry

  The Journal of Biochemistry 121 (6), 1123-1128, 1997

  The Japanese Biochemical Society

キーワード

• hepatitis delta virus
• metal binding
• oligonucleotide
• ribozyme
• RNA

詳細情報

• CRID: 1573668927906617728
• NII論文ID: 130003417722
• ISSN: 0021924X
• Web Site: https://jlc.jst.go.jp/DN/JALC/00083828638?from=CiNii
• 本文言語コード: en
• データソース種別:
  • CiNii Articles