Modification of Proteoglycan Synthesis by Corneal Stromal Cells on Co-Culture with Either Epithelial or Endothelial Cells.
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- Nakazawa Kiyoshi
- Section of Radiochemistry Meijo University
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- Takahashi Ikuko
- Radio-isotope Center, Faculty of Pharmacy, Meijo University
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- Ohno Yuko
- Section of Radiochemistry Meijo University
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- Sato Masami
- Section of Radiochemistry Meijo University
抄録
Corneal stromal cells, prepared from 2-day-old chicks and embedded in collagen gel on an insert dish, were co-cultured with corneal epithelial cells or endothelial cells on a fibronectin-coated companion plate. Cell growth and proteoglycan synthesis, as determined as the incorporation of [35S] sulfate and [3H]glucosamine, were examined for each cell type in both combinations. In comparison with single cultures, growth was affected little, while proteoglycan synthesis was appreciably altered in both combinations. Stromal and epithelial cells stimulated proteoglycan synthesis by each other, while endothelial cells stimulated the synthesis by stromal cells, but stromal cells reduced that by endothelial cells to some extent. Endothelial cells alone synthesized proteoglycans much more actively than did epithelial or stromal cells. Analysis of the radiolabeled proteoglycans revealed that endothelial and epithelial cells had different effects on proteoglycan synthesis by stromal cells: the former tended to increase keratan sulfate synthesis by stromal cells, while the latter tended to increase chondroitin sulfate/dermatan sulfate synthesis. Proteoglycan synthesis in the corneal stroma in vivo might thus be controlled by the balance between the antipodal actions of the epithelial and endothelial cell layers.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 122 (4), 851-858, 1997
The Japanese Biochemical Society
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キーワード
詳細情報 詳細情報について
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- CRID
- 1572261553023045248
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- NII論文ID
- 130003417755
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- ISSN
- 0021924X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles