肝蛭成虫抗原のEITB法による解析

• 板垣 匡

• 坂本 司

• 板垣 博

書誌事項

タイトル別名

• Analysis of Fasciola sp. Antigen by Enzyme-Linked Immunotransfer Blot Using Sera from Experimentally and Naturally Infected Cattle.

抄録

Soluble polypeptides extracted from adult Fasciola sp. were analysed by SDS-PAGE, and their antigenicity was characterized by enzyme-linked immunotransfer blot (EITB) using sera from experimentally and naturally infected cattle. Polypeptides of adult Fasciola sp. with estimated molecular mass of 64-52 kDa, 38-28 kDa, 17 kDa, 15 kDa, 13 kDa and 12 kDa, were recognized by sera obtained in both early and late stages of infection. Furthermore, two polypeptides of more than 160 kDa were detected by sera only in the early stage of infection. The results of EITB using sera of dairy cows naturally infected with Fasciola sp. suggest that polypeptides of 64-52 kDa may be candidates as serodiagnostic antigen of bovine fascioliasis.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 57 (3), 511-513, 1995

  公益社団法人 日本獣医学会

キーワード

• bovine fascioliasis
• enzyme-linked immunotransfer blot
• Fasciola antigen

詳細情報

• CRID: 1390001206427296256
• NII論文ID: 130003426364
• DOI: 10.1292/jvms.57.511
• ISSN: 13477439
• ISSN: 09167250
• PubMed: 7548408
• Web Site: http://www.jstage.jst.go.jp/article/jvms1991/57/3/57_3_511/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可