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- KURAMOCHI Gen
- Department of Medicine (II), Niigata University School of Medicine
抄録
Effect of exogenous D-glucose on oxygen consumption (QO<sub>2</sub>) was investigated in the proximal tubules of the Triturus kidney. In the proximal tubule, which was perfused and incubated with a solution containing Na+, QO<sub>2</sub> was increased dependently of the concentration of added glucose. By removal of Na+ from the perfusate and incubation solutions, QO<sub>2</sub> kept the level even by increasing adding glucose. The glucose dependence of QO<sub>2</sub> in the proximal tubule showed the saturable manner of a Michaelis-Menten type and gave a half saturation concentration of 1.3mM. When phlorizin (1×10-3M) or ouabain (1×10-3M) was added to the perfusate and incubation solutions, the glucose dependency of QO<sub>2</sub> was abolished, even in the Na+-containing solution. When α-methyl-D-glucoside (α-MG) was used instead of D-glucose, the QO<sub>2</sub> of the proximal tubule showed a dependency on the concentration of α-MG. On the other hand, when D-mannitol was used instead of D-glucose, the QO<sub>2</sub> was independent on the concentration of D-mannitol. The increase in QO<sub>2</sub> of the proximal tubule by exogenous D-glucose could be explained by the stimulation of the Na+ pump, which is the consequence of enhanced Na+ entry through Na+/D-glucose cotransport across the luminal membrane.
収録刊行物
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- The Japanese Journal of Physiology
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The Japanese Journal of Physiology 36 (2), 287-293, 1986
一般社団法人 日本生理学会
